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Volume 18, Number 10—October 2012
Research

WU and KI Polyomaviruses in Respiratory Samples from Allogeneic Hematopoietic Cell Transplant Recipients

Jane Kuypers1Comments to Author , Angela P. Campbell1, Katherine A. Guthrie, Nancy L. Wright, Janet A. Englund, Lawrence Corey, and Michael Boeckh
Author affiliations: University of Washington, Seattle, Washington, USA (J. Kuypers, A.P. Campbell, N.L. Wright, J.A. Englund, L. Corey, M. Boeckh); Fred Hutchinson Cancer Research Center, Seattle (J. Kuypers, A.P. Campbell, K.A. Guthrie, J.A. Englund, L. Corey, M. Boeckh); and Seattle Children’s Hospital, Seattle (A.P. Campbell, J.A. Englund)

Main Article

Table 2

Characteristics of 222 HCT recipients tested for KIPyV or WUPyV*

Characteristic No. (%) recipients
All, n = 222 Infected with either virus, n = 62 Not infected, n = 160
Age, y

<20

23 (10) 18 (29) 5 (3)

20–39

37 (17) 14 (23) 23 (14)

40–59

99 (45) 17 (27) 82 (51)

>60

63 (28) 13 (21) 50 (31)
Sex

F

87 (39) 23 (37) 64 (40)

M

135 (61) 39 (63) 96 (60)
Underlying disease risk†

Standard

142 (64) 40 (65) 102 (64)

High

80 (36) 22 (35) 58 (36)
Stem cell source

Bone marrow

28 (13) 12 (19) 16 (10)

Peripheral blood

179 (81) 47 (76) 132 (83)

Cord blood

15 (7) 3 (5) 12 (8)
CMV serostatus

D+/R+

56 (25) 17 (27) 39 (24)

D+/R–

81 (36) 22 (35) 59 (37)

D–/R+

16 (7) 4 (6) 12 (8)

D–/R–

69 (31) 19 (31) 50 (31)
Donor match

Related–matched

77 (35) 23 (37) 54 (34)

Related–mismatched

9 (4) 6 (10) 3 (2)

Unrelated–cord blood

15 (7) 3 (5) 12 (8)

Unrelated–matched

97 (44) 24 (39) 73 (46)

Unrelated–mismatched

24 (11) 6 (10) 18 (11)
Conditioning regimen

Myeloablative

128 (58) 41 (66) 87 (54)

Nonmyeloablative

94 (42) 21 (34) 73 (46)
Acute graft-versus-host disease

Grade 0 or 1

78 (35) 30 (48) 48 (30)

Grade 2–4

144 (65) 32 (52) 112 (70)

*HCT, hematopoietic cell transplantation; KIPyV, KI polyomavirus; WUPyV, WU polyomavirus; CMV, cytomegalovirus; D, donor; R, recipient.
†The underlying disease stage of a patient was categorized as low, intermediate, or high risk (29,31).

Main Article

References
  1. Allander  T, Andreasson  K, Gupta  S, Bjerkner  A, Bogdanovic  G, Persson  MA, Identification of a third human polyomavirus. J Virol. 2007;81:41306. DOIPubMedGoogle Scholar
  2. Gaynor  AM, Nissen  MD, Whiley  DM, Mackay  IM, Lambert  SB, Wu  G, Identification of a novel polyomavirus from patients with acute respiratory tract infections. PLoS Pathog. 2007;3:e64. DOIPubMedGoogle Scholar
  3. Bialasiewicz  S, Whiley  DM, Lambert  SB, Wang  D, Nissen  MD, Sloots  TP. A newly reported human polyomavirus, KI virus, is present in the respiratory tract of Australian children. J Clin Virol. 2007;40:158. DOIPubMedGoogle Scholar
  4. Le  BM, Demertzis  LM, Wu  G, Tibbets  RJ, Buller  R, Arens  MQ, Clinical and epidemiologic characterization of WU polyomavirus infection, St. Louis, Missouri. Emerg Infect Dis. 2007;13:19368. DOIPubMedGoogle Scholar
  5. Norja  P, Ubillos  I, Templeton  K, Simmonds  P. No evidence for an association between infections with WU and KI polyomaviruses and respiratory disease. J Clin Virol. 2007;40:30711. DOIPubMedGoogle Scholar
  6. Han  TH, Chung  JY, Koo  JW, Kim  SW, Hwang  ES. WU polyomavirus in children with acute lower respiratory tract infections, South Korea. Emerg Infect Dis. 2007;13:17668. DOIPubMedGoogle Scholar
  7. Ren  L, Gonzalez  R, Xie  Z, Zhang  J, Liu  C, Li  J, WU and KI polyomavirus present in the respiratory tract of children, but not in immunocompetent adults. J Clin Virol. 2008;43:3303. DOIPubMedGoogle Scholar
  8. Wattier  RL, Vazquez  M, Weibel  C, Shapiro  ED, Ferguson  D, Landry  ML, Role of human polyomaviruses in respiratory tract disease in young children. Emerg Infect Dis. 2008;14:17668. DOIPubMedGoogle Scholar
  9. Hormozdi  DJ, Arens  MQ, Le  BM, Buller  RS, Agapov  E, Storch  GA. KI polyomavirus detected in respiratory tract specimens from patients in St. Louis, Missouri. Pediatr Infect Dis J. 2010;29:32933.PubMedGoogle Scholar
  10. Neske  F, Blessing  K, Ullrich  F, Prottel  A, Wolfgang Kreth  H, Weissbrich  B. WU polyomavirus infection in children, Germany. Emerg Infect Dis. 2008;14:6801. DOIPubMedGoogle Scholar
  11. Yuan  XH, Jin  Y, Xie  ZP, Gao  HC, Xu  ZQ, Zheng  LS, Prevalence of human KI and WU polyomaviruses in children with acute respiratory tract infection in China. J Clin Microbiol. 2008;46:35225. DOIPubMedGoogle Scholar
  12. Abed  Y, Wang  D, Boivin  G. WU polyomavirus in children, Canada. Emerg Infect Dis. 2007;13:193941. DOIPubMedGoogle Scholar
  13. van de Pol  AC, Wolfs  TF, Jansen  NJ, Kimpen  JL, van Loon  AM, Rossen  JW. Human bocavirus and KI/WU polyomaviruses in pediatric intensive care patients. Emerg Infect Dis. 2009;15:4547. DOIPubMedGoogle Scholar
  14. Boothpur  R, Brennan  DC. Human polyoma viruses and disease with emphasis on clinical BK and JC. J Clin Virol. 2010;47:30612. DOIPubMedGoogle Scholar
  15. Tan  CS, Koralnik  IJ. Progressive multifocal leukoencephalopathy and other disorders caused by JC virus: clinical features and pathogenesis. Lancet Neurol. 2010;9:42537. DOIPubMedGoogle Scholar
  16. zur Hausen  H. Novel human polyomaviruses: re-emergence of a well known virus family as possible human carcinogens. Int J Cancer. 2008;123:24750. DOIPubMedGoogle Scholar
  17. Sharp  CP, Norja  P, Anthony  I, Bell  JE, Simmonds  P. Reactivation and mutation of newly discovered WU, KI, and Merkel cell carcinoma polyomaviruses in immunosuppressed individuals. J Infect Dis. 2009;199:398404. DOIPubMedGoogle Scholar
  18. Bialasiewicz  S, Whiley  DM, Lambert  SB, Nissen  MD, Sloots  TP. Detection of BK, JC, WU, or KI polyomaviruses in faecal, urine, blood, cerebrospinal fluid and respiratory samples. J Clin Virol. 2009;45:24954. DOIPubMedGoogle Scholar
  19. Debiaggi  M, Canducci  F, Brerra  R, Sampaolo  M, Marinozzi  MC, Parea  M, Molecular epidemiology of KI and WU polyomaviruses in infants with acute respiratory disease and in adult hematopoietic stem cell transplant recipients. J Med Virol. 2010;82:1536. DOIPubMedGoogle Scholar
  20. Mourez  T, Bergeron  A, Ribaud  P, Scieux  C, de Latour  RP, Tazi  A, Polyomaviruses KI and WU in immunocompromised patients with respiratory disease. Emerg Infect Dis. 2009;15:1079. DOIPubMedGoogle Scholar
  21. Rao  S, Garcea  RL, Robinson  CC, Simões  EA. WU and KI polyomavirus infections in pediatric hematology/oncology patients with acute respiratory tract illness. J Clin Virol. 2011;52:2832. DOIPubMedGoogle Scholar
  22. Kuypers  J, Campbell  AP, Cent  A, Corey  L, Boeckh  M. Comparison of conventional and molecular detection of respiratory viruses in hematopoietic cell transplant recipients. Transpl Infect Dis. 2009;11:298303. DOIPubMedGoogle Scholar
  23. Bialasiewicz  S, Whiley  DM, Lambert  SB, Gould  A, Nissen  MD, Sloots  TP. Development and evaluation of real-time PCR assays for the detection of the newly identified KI and WU polyomaviruses. J Clin Virol. 2007;40:914. DOIPubMedGoogle Scholar
  24. Foulongne  V, Olejnik  Y, Perez  V, Elaerts  S, Rodiere  M, Segondy  M. Human bocavirus in French children. Emerg Infect Dis. 2006;12:12513. DOIPubMedGoogle Scholar
  25. Kuypers  J, Wright  N, Corey  L, Morrow  R. Detection and quantification of human metapneumovirus in pediatric specimens by real-time RT-PCR. J Clin Virol. 2005;33:299305. DOIPubMedGoogle Scholar
  26. Kuypers  J, Wright  N, Ferrenberg  J, Huang  ML, Cent  A, Corey  L, Comparison of real-time PCR assays with fluorescent-antibody assays for diagnosis of respiratory virus infections in children. J Clin Microbiol. 2006;44:23828. DOIPubMedGoogle Scholar
  27. Kuypers  J, Wright  N, Morrow  R. Evaluation of quantitative and type-specific real-time RT-PCR assays for detection of respiratory syncytial virus in respiratory specimens from children. J Clin Virol. 2004;31:1239. DOIPubMedGoogle Scholar
  28. Lu  X, Holloway  B, Dare  RK, Kuypers  J, Yagi  S, Williams  JV, Real-time reverse transcription–PCR assay for comprehensive detection of human rhinoviruses. J Clin Microbiol. 2008;46:5339. DOIPubMedGoogle Scholar
  29. Milano  F, Campbell  AP, Guthrie  KA, Kuypers  J, Englund  JA, Corey  L, Human rhinovirus and coronavirus detection among allogeneic hematopoietic stem cell transplantation recipients. Blood. 2010;115:208894. DOIPubMedGoogle Scholar
  30. Kuypers  J, Martin  ET, Heugel  J, Wright  N, Morrow  R, Englund  JA. Clinical disease in children associated with newly described coronavirus subtypes. Pediatrics. 2007;119:e706. DOIPubMedGoogle Scholar
  31. Koc  S, Leisenring  W, Flowers  ME, Anasetti  C, Deeg  HJ, Nash  RA, Therapy for chronic graft-versus-host disease: a randomized trial comparing cyclosporine plus prednisone versus prednisone alone. Blood. 2002;100:4851. DOIPubMedGoogle Scholar
  32. Abedi Kiasari  B, Vallely  PJ, Corless  CE, Al-Hammadi  M, Klapper  PE. Age-related pattern of KI and WU polyomavirus infection. J Clin Virol. 2008;43:1235. DOIPubMedGoogle Scholar
  33. van der Zalm  MM, Rossen  JW, van Ewijk  BE, Wilbrink  B, van Esch  PC, Wolfs  TF, Prevalence and pathogenicity of WU and KI polyomaviruses in children, the Netherlands. Emerg Infect Dis. 2008;14:17879. DOIPubMedGoogle Scholar
  34. Kean  JM, Rao  S, Wang  M, Garcea  RL. Seroepidemiology of human polyomaviruses. PLoS Pathog. 2009;5:e1000363. DOIPubMedGoogle Scholar
  35. Nguyen  NL, Le  BM, Wang  D. Serologic evidence of frequent human infection with WU and KI polyomaviruses. Emerg Infect Dis. 2009;15:1199205. DOIPubMedGoogle Scholar
  36. Babakir-Mina  M, Ciccozzi  M, Bonifacio  D, Bergallo  M, Costa  C, Cavallo  R, Identification of the novel KI and WU polyomaviruses in human tonsils. J Clin Virol. 2009;46:759. DOIPubMedGoogle Scholar
  37. Kantola  K, Sadeghi  M, Lahtinen  A, Koskenvuo  M, Aaltonen  LM, Mottonen  M, Merkel cell polyomavirus DNA in tumor-free tonsillar tissues and upper respiratory tract samples: implications for respiratory transmission and latency. J Clin Virol. 2009;45:2925. DOIPubMedGoogle Scholar
  38. Bialasiewicz  S, Whiley  DM, Lambert  SB, Jacob  K, Bletchly  C, Wang  D, Presence of the newly discovered human polyomaviruses KI and WU in Australian patients with acute respiratory tract infection. J Clin Virol. 2008;41:638. DOIPubMedGoogle Scholar
  39. Neske  F, Blessing  K, Prottel  A, Ullrich  F, Kreth  HW, Weissbrich  B. Detection of WU polyomavirus DNA by real-time PCR in nasopharyngeal aspirates, serum, and stool samples. J Clin Virol. 2009;44:1158. DOIPubMedGoogle Scholar
  40. Babakir-Mina  M, Ciccozzi  M, Alteri  C, Polchi  P, Picardi  A, Greco  F, Excretion of the novel polyomaviruses KI and WU in the stool of patients with hematological disorders. J Med Virol. 2009;81:166873. DOIPubMedGoogle Scholar

Main Article

1These authors contributed equally to this article.

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Page updated: September 13, 2012
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