Volume 18, Number 3—March 2012
Research
Pathogenic Potential to Humans of Bovine Escherichia coli O26, Scotland
Table 2
Primers and PCR conditions for Escherichia coli O26, Scotland
| Primer name | Primer sequence, 5′ → 3′ | Target gene | Annealing | Amplicon size, bp | Reference |
|---|---|---|---|---|---|
| stx1F stx1R | ATAAATCGCCATTCGTTGACTAC AGAACGCCCACTGAGATCATC | stx1 | 60°C,45 s | 180 | (24) |
| stx2F stx2R | GGCACTGTCTGAAACTGCTCC TCGCCAGTTATCTGACATTCTG | stx2 | 60°C,45 s | 255 | (24) |
| eaeAF eaeAR | GACCCGGCACAAGCATAAGC CCACCTGCAGCAACAAGAGG | eae | 60°C,45 s | 384 | (24) |
| hlyAF hlyAR | GCATCATCAAGCGTACGTTCC AATGAGCCAAGCTGGTTAAGCT | hlyA | 60°C,45 s | 534 | (24) |
| sepLF sepLR | GCTAAGCCTGGGATATCGC ACAATCGATACCCGAGAAGG | sepL | 60°C,45 s | 725 | This study |
| univ tccP/tccp2-F tccP-R | GTAAAAACCAGCTCACCTTTTTC TCACGAGCGCTTAGATGTATTAAT | tccp tccP2 | 64°C,60 s | Variable | (25) |
| espAF espAR | CCTTCTCGGGTATCGATTGTCG CAGAGGGCGTCACTAATGAGTG | espA | 58°C,60 s | 1012 | This study |
| LEE1promF LEE1promR | CGAATGGTACGGTTATGCGGG GCTCTCGCAGTCGCTTTGCTTCC | LEE1 | 58°C,60 s | 645 | This study |


