Figure. . Phylogenetic relationships among genotypes 1–4, wild boar, rabbit, avian (chicken), and rat hepatitis E virus (HEV) isolates. The nucleic acid sequence alignment was performed by using ClustalX 1.81 (www.clustal.org). The genetic distance was calculated by the Kimura 2-parameter method. A phylogenetic tree with 1,000 bootstrap replicates was generated by the neighbor-joining method, based on the entire genome (A) and open reading frame 3 (B) of the genotypes 1–4, wild boar, rabbit, avian (chicken), and rat HEV isolates. Scale bar indicates nucleotide substitutions per site. Boldface indicates isolates used in this study.