Foodborne Transmission of Bovine Spongiform Encephalopathy to Nonhuman Primates
Figure 3. . Western immunoblot analysis of proteinease-resistant prion protein (PrPres) (from brain). The size of the nonglycosylated band was either 21 kDa (termed type 1, sCJD case BN141) or 19 kDa (termed type 2, sCJD case BN144). Diglycosylated PrPres predominated in the BSE inoculum and in all macaques showing neurologic signs (macaque S4 is shown as a representative example for all symptomatic cases), and the pattern is termed type 2B to distinguish it from type 2 cases in which the monoglycosylated form predominated (Type 2A, sCJD case BN144). Two different mAbs were used for routine immunodetection: 6H4 (left panel) and 3F4 (right panel). Blots were co-stained with a polyclonal anti-actin antiserum. BSE, bovine spongiform encephalopathy; sCJD, sporadic Creutzfeldt-Jakob disease; PK, proteinase K; mAb, monoclonal antibody.