Emerging Infectious Disease ISSN: 1080-6059

Two Morbilliviruses Implicated in Bottlenose Dolphin Epizootics

Jeffery K. Taubenberger*, Mark Tsai*, Amy E. Krafft*, Jack H. Lichy*, Ann H. Reid*, F. Yvonne Schulman†, and Thomas P. Lipscomb†

Author affiliations: *Division of Molecular Pathology, Department of Cellular Pathology; †Department of Veterinary Pathology, Armed Forces Institute of Pathology,Washington, DC 20306-6000 USA

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Figure

Partial nucleotide sequence of DMV and PMV morbillivirus P gene compared with published DMV and PMV sequences (12). Primer sequences used were 5'-ATC TGC TCC CAG GAT TAA GGT CGA-3' (forward) and 5'-CGG GAT TGG TGG GAC CTT TA-3' (reverse). RT- PCR was performed (11). PCR products were cycle sequenced (20) or cloned into PDK101 (21) and sequenced by using T7 Sequenase (Amersham Corporation, Arlington Heights, IL) according to the manufacturer’s instructions.

Figure. Partial nucleotide sequence of DMV and PMV morbillivirus P gene compared with published DMV and PMV sequences (12). Primer sequences used were 5'-ATC TGC TCC CAG GAT TAA GGT CGA-3' (forward) and 5'-CGG GAT TGG TGG GAC CTT TA-3' (reverse). RT- PCR was performed (11). PCR products were cycle sequenced (20) or cloned into PDK101 (21) and sequenced by using T7 Sequenase (Amersham Corporation, Arlington Heights, IL) according to the manufacturer’s instructions.

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