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Volume 22, Number 9—September 2016
Research

Staphylococcus aureus Regulatory RNAs as Potential Biomarkers for Bloodstream Infections

Valérie Bordeau, Anne Cady, Matthieu Revest, Octavie Rostan, Mohamed Sassi, Pierre Tattevin, Pierre-Yves Donnio, and Brice FeldenComments to Author 
Author affiliations: University of Rennes 1, Rennes, France (V. Bordeau, A. Cady, M. Revest, O. Rostan, M. Sassi, P. Tattevin, P.-Y. Donnio, B. Felden); Institut National de la Santé et de la Recherche Médicale Unité 835, Rennes (V. Bordeau, M. Sassi, P. Tattevin, B. Felden); Rennes University Hospital, Rennes (A. Cady, M. Revest, P. Tattevin, P.-Y. Donnio)

Main Article

Figure 1

Phylogenetic and molecular typing of Staphylococcus aureus by using small RNAs (SRNAs), Rennes, France. A) Phylogenetic tree showing sRNA gene content and expression. Genotyping by multilocus sequence typing (MLST) and the maximum-parsimony method classified 83 S. aureus isolates (40 from patients with bloodstream infections and 40 from nasal carriers). Strains were distributed into 17 sequence types (STs) in groups 1 and 2 according to the method of Cooper and Feil (16). Three isolates were not

Figure 1. Phylogenetic and molecular typing of Staphylococcus aureus by using small RNAs (SRNAs), Rennes, France. A) Phylogenetic tree showing sRNA gene content and expression. Genotyping by multilocus sequence typing (MLST) and the maximum-parsimony method classified 83 S. aureus isolates (40 from patients with bloodstream infections and 40 from nasal carriers). Strains were distributed into 17 sequence types (STs) in groups 1 and 2 according to the method of Cooper and Feil (16). Three isolates were not included in the tree because 2 could not be typed and 1 belonged to the divergent ST883 lineage. Numbers indicate genetic distances between nodes. Circle sizes are proportional to number of isolates, and colors indicate origin. B) sRNA gene distribution among 83 analyzed strains. Five sRNAs were from pathogenicity islands and 3 sRNAs were from the core genome. Red indicates presence of sRNA and white indicates absence of SRNA. Heat map was constructed by using R software (https://www.r-project.org/). tmRNA, transfer–messenger RNA; ND, not determined.

Main Article

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Page created: August 23, 2016
Page updated: August 23, 2016
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