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Volume 22, Number 9—September 2016
Research

Staphylococcus aureus Regulatory RNAs as Potential Biomarkers for Bloodstream Infections

Valérie Bordeau, Anne Cady, Matthieu Revest, Octavie Rostan, Mohamed Sassi, Pierre Tattevin, Pierre-Yves Donnio, and Brice FeldenComments to Author 
Author affiliations: University of Rennes 1, Rennes, France (V. Bordeau, A. Cady, M. Revest, O. Rostan, M. Sassi, P. Tattevin, P.-Y. Donnio, B. Felden); Institut National de la Santé et de la Recherche Médicale Unité 835, Rennes (V. Bordeau, M. Sassi, P. Tattevin, B. Felden); Rennes University Hospital, Rennes (A. Cady, M. Revest, P. Tattevin, P.-Y. Donnio)

Main Article

Figure 5

Expression levels of Sbi and SaeR proteins of Staphylococcus aureus isolated from patients with bloodstream infections and asymptomatic carriers, Rennes, France. A) Western blot of Sbi protein for 61 strains during early exponential growth phase. A protein sample from strain 19 was loaded on each gel and used as an internal control to prevent intensity variations of the bands between each experiment. Carriage: lane 1, 1; lane 2, 10; lane 3, 15; lane 4, 19; lane 5, 102; lane 6, 1911; lane 7, 0026

Figure 5. Expression levels of Sbi and SaeR proteins of Staphylococcus aureus isolated from patients with bloodstream infections and asymptomatic carriers, Rennes, France. A) Western blot of Sbi protein for 61 strains during early exponential growth phase. A protein sample from strain 19 was loaded on each gel and used as an internal control to prevent intensity variations of the bands between each experiment. Carriage: lane 1, 1; lane 2, 10; lane 3, 15; lane 4, 19; lane 5, L102; lane 6, 1911; lane 7, 0026; lane 8, 0310; lane 9; 2203; lane 10, 2155; lane 11, 2752; lane 12, 2167; lane 13, 1954; lane 14, 1955; lane 15, 21; lane 16, 11; lane 17, L45; lane 18, 8; lane 19, 23. Sepsis: lane 1, 49; lane 2, 18; lane 3, 88; lane 4, 82; lane 5, 46; lane 6, 71; lane 7, 59; lane 8, 75; lane 9; 91; lane 10, 86; lane 11, 9; lane 12, 43; lane 13, 20; lane 14, 52; lane 15, 85; lane 16, 104; lane 17, 54; lane 18, 58; lane 19, 61; lane 20, 101; lane 21, 62; lane 22, 74. Shock: lane 1, 26; lane 2, 44; lane 3, 17; lane 4, 14; lane 5, 42; lane 6, 30; lane 7, 34; lane 8, 45; lane 9, 57; lane 10, 89; lane 11, 27; lane 12, 11; lane 13, 28; lane 14, 430; lane 15, 069; lane 16, 886. B) and C) Sbi/total protein. D) SaeR/total protein. SaeR is a positive regulator of Sbi protein. p values (by Mann-Whitney U test) for significant differences are shown. Experiments were performed in triplicate. Horizontal lines indicate medians. To exclude loading variations between samples, values were normalized against total protein levels. AU, arbitrary units; Sbi, second immunoglobulin-binding.

Main Article

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Page updated: August 23, 2016
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