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Volume 3, Number 4—December 1997
THEME ISSUE
Foodborne

Controlling Emerging Foodborne Microbial Hazards

Epidemiology and Detection as Options for Control of Viral and Parasitic Foodborne Disease

Lee-Ann JaykusComments to Author 
Author affiliation: North Carolina State University, Raleigh, North Carolina, USA

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Table 2

Emerging detection methods for human enteric viruses in foods

Field
Pathogen Sample Detection limit app. Ref.
Nucleic Acid Extraction/RT-PCR
HAV Clams 2000 particles/g
(10 PFU/g) No 44
Poliovirus Oysters 38 PFU/20 g
(2 PFU/g) No 42
HAV Clams/Oysters 100 PFU/1.5 g
(67 PFU/g) No 43
Norwalk Clams/Oysters 5-10 PCRU/1.5 g
(3-7 PCRU/g) No 43
Poliovirus Oysters/Mussels 10 PFU/5 g
(2 PFU/g) No 45
SRSV Oysters/Mussels Not specified Yes 46
Polivirus,
HAV Clams 100 PFU/50 g
(2 PFU/g) No 50
Norwalk Clams 1000 PCRU/50 g
(20 PCRU/g) No 50
Norwalk Various 20-200 PCRU/10 g
(2-20 PCRU/g) No 47
Antibody-Capture/RT-PCR
HAV Clams/Oysters Not specified No 48
HAV Oysters Not specified Yes 19
Virion Concentration
Poliovirus,
HAV Oysters 10 PFU/50 g
(0.02 PFU/g) Yes 49,
Norwalk Oysters 4500 PCRU/50 g
(90 PCRU/g) No 49,51
Poliovirus,
HAV Clams 1000 PFU/50 g
(20 PFU/g) No 50
Norwalk Clams 100 PCRU/50 g
(2 PCRU/g) No 50

HAV = hepatitis A virus; RT-PCR = reverse transcriptase polymerase chain reaction; PCRU = PCR-amplifiable units; SRSV = small round-structured gastrointestinal virus.

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