Volume 3, Number 4—December 1997
THEME ISSUE
Foodborne
Controlling Emerging Foodborne Microbial Hazards
Epidemiology and Detection as Options for Control of Viral and Parasitic Foodborne Disease
Lee-Ann Jaykus
Table 2
Emerging detection methods for human enteric viruses in foods
| Field | ||||
|---|---|---|---|---|
| Pathogen | Sample | Detection limit | app. | Ref. |
| Nucleic Acid | Extraction/RT-PCR | |||
| HAV | Clams | 2000 particles/g (10 PFU/g) | No | 44 |
| Poliovirus | Oysters | 38 PFU/20 g (2 PFU/g) | No | 42 |
| HAV | Clams/Oysters | 100 PFU/1.5 g (67 PFU/g) | No | 43 |
| Norwalk | Clams/Oysters | 5-10 PCRU/1.5 g (3-7 PCRU/g) | No | 43 |
| Poliovirus | Oysters/Mussels | 10 PFU/5 g (2 PFU/g) | No | 45 |
| SRSV | Oysters/Mussels | Not specified | Yes | 46 |
| Polivirus, HAV | Clams | 100 PFU/50 g (2 PFU/g) | No | 50 |
| Norwalk | Clams | 1000 PCRU/50 g (20 PCRU/g) | No | 50 |
| Norwalk | Various | 20-200 PCRU/10 g (2-20 PCRU/g) | No | 47 |
| Antibody-Capture/RT-PCR | ||||
| HAV | Clams/Oysters | Not specified | No | 48 |
| HAV | Oysters | Not specified | Yes | 19 |
| Virion Concentration | ||||
| Poliovirus, HAV | Oysters | 10 PFU/50 g (0.02 PFU/g) | Yes | 49, |
| Norwalk | Oysters | 4500 PCRU/50 g (90 PCRU/g) | No | 49,51 |
| Poliovirus, HAV | Clams | 1000 PFU/50 g (20 PFU/g) | No | 50 |
| Norwalk | Clams | 100 PCRU/50 g (2 PCRU/g) | No | 50 |
HAV = hepatitis A virus; RT-PCR = reverse transcriptase polymerase chain reaction; PCRU = PCR-amplifiable units; SRSV = small round-structured gastrointestinal virus.
