Volume 3, Number 4—December 1997
THEME ISSUE
Foodborne
Controlling Emerging Foodborne Microbial Hazards
Epidemiology and Detection as Options for Control of Viral and Parasitic Foodborne Disease
Lee-Ann Jaykus
Table 3
Emerging detection methods for parasitic protozoa in water
| Pathogen | Detection limit | Viability | Differentiation | Ref. |
|---|---|---|---|---|
| Flow Cytometry with Fluorescent Imaging and CCD | ||||
| Cryptosporidium | NR | Yes, using differential fluorogenic vital dyes | NR | 58,59 |
| DNA Hybridization | ||||
| Giardia (16s-like rDNA) | 1cyst | NR | No | 60 |
| Giardia (16s-like rDNA) | NR | NR | Yes-in situ hybridization with differential fluorescence G. lamblia; G. muris | 61 |
| Polymerase Chain Reaction | ||||
| Giardia (Giardin gene) | 1 cyst | Partial, using mRNA as target; Depends on inactivation method | NR | 62 |
| Giardia (Giardin gene) | 1 cyst | NR | Yes-primer sequence G. duodenalis | 63 |
| Cryptosporidium (Target not specified) | 20 oocysts | No | Yes-by hybridization C. parvum | 64 |
CCD = cooled charge couple device; NR = not reported
