Figure 3. Comparative Western blot analyses with Sha31 and 12B2 monoclonal antibodies (mAbs) of brain protease-resistant prion protein (PrPres) from BSE-H–infected mice. Mice infected with isolate 07-644 (lane 1), 02-2695 (lanes 2 and 3), or 45 (lanes 4 and 5) at first passage showing either high-type (lanes 1, 2, and 4) or classical BSE–like PrPres molecular profile (lanes 3 and 5). Panel A was shown with Sha31 mAb; panel B was shown with 12B2 mAb. The same quantities of PrPres were loaded in both panels A and B. Values to the left indicate molecular mass in kDa. BSE, bovine spongiform encephalopathy; BSE-H, unglycosylated PrPres that is higher than classical BSE.