Figure 5. Comparison of the amount of protease-resistant prion protein (PrPres) in brain sample from mouse inoculated with BSE-H (isolate 02-2695) showing either high-type (lane 1 and 2, first and second passages, respectively) or classical BSE–like PrPres molecular profile (lanes 3 and 4, first and second passages, respectively). Identical amounts of 10% brain homogenate were loaded in each lane. Western blot was shown with Sha31 monoclonal antibody. Values to the left indicate molecular mass in kDa. BSE, bovine spongiform encephalopathy; BSE-H, unglycosylated PrPres that is higher than classical BSE–like.