Author affiliations: Author affiliations: Centro de Investigación en Sanidad Animal, Madrid, Spain (J.-M. Torres, I. Prieto, P. Lorenzo, M. Larska, J.-C. Espinosa); Ecole Nationale Vétérinaire de Toulouse, Toulouse, France (O. Andréoletti, C. Lacroux); Agence Francaise de Sécurité Sanitaire des Aliments, Lyon, France (T. Baron)
Figure 5. Comparison of the amount of protease-resistant prion protein (PrPres) in brain sample from mouse inoculated with BSE-H (isolate 02-2695) showing either high-type (lane 1 and 2, first and second passages, respectively) or classical BSE–like PrPres molecular profile (lanes 3 and 4, first and second passages, respectively). Identical amounts of 10% brain homogenate were loaded in each lane. Western blot was shown with Sha31 monoclonal antibody. Values to the left indicate molecular mass in kDa. BSE, bovine spongiform encephalopathy; BSE-H, unglycosylated PrPres that is higher than classical BSE–like.
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