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Volume 18, Number 12—December 2012

Research

Transmission Routes for Nipah Virus from Malaysia and Bangladesh

Bronwyn A. Clayton, Deborah Middleton, Jemma Bergfeld, Jessica Haining, Rachel Arkinstall, Linfa Wang, and Glenn A. MarshComments to Author 
Author affiliations: Author affiliations: Commonwealth Scientific and Industrial Research Organisation Livestock Industries, Geelong, Victoria, Australia (B.A. Clayton, D. Middleton, J. Bergfeld, J. Haining, R. Arkinstall, L. Wang, G.A. Marsh); University of Melbourne, Parkville, Victoria, Australia (B.A. Clayton); Duke–National University of Singapore Graduate Medical School, Singapore (L. Wang)

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Table 4

NiV in environmental samples after experimental infection of 15 ferrets*

Virus strain, cage no. (ferret no.) log10 NiV copies/mL†
Dpi 1
Dpi 2
Dpi 3
Dpi 4
Dpi 5
Dpi 6
Dpi 7
Dpi 8
Dpi 9
Dpi 10
U F U F U F U F U F U F U F U F U F U F
Bangladesh

1 (B1, B2)

5.1‡ NA NA NA NA NA NA

2 (B3, B4)

NS NS 5.2‡ 4.1 NA NA NA NA NA NA

3 (B5, B6)

4.1 3.4 4.6‡ 5.3‡ 4.6 NA NA

4 (B7, B8)




















3.6
4.3


4.9‡

NS


NA
NA
Malaysia NA NA

5 (M9)

4.9‡ NA NA NA NA NA NA

6 (M10, 11)

3.6‡ 4.9‡ NA NA NA NA NA NA

7 (M12, 13)

5.1‡ 4.9‡ NA NA

8 (M14, 15)

4.6‡ 3.6

*NiV, Nipah virus; dpi, days postinfection; U, urine; F, feces; –,negative; NA, not applicable because cage was empty after euthanasia of ferrets; NS, no sample available.
†Calculated from standard curve generated for NiV N gene copies by reverse transcription PCR. Samples with mean cycle threshold ≤39.1 (based on duplicate reactions) were defined as NiV positive.
‡Sample was also NiV positive by virus isolation.

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