Author affiliations: Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain (J.-M. Torres, J. Castilla, B. Pintado, A. Gutiérrez-Adán P. Aguilar-Calvo, A.-I. Arroba, B. Parra-Arrondo, J.-C. Espinosa); Basque Foundation for Science, Bilbao, Spain (J. Castilla); Ecole Nationale Vétérinaire de Toulouse, Toulouse, France (O. Andréoletti); Hospitalet de Llobregat, Barcelona, Spain (I. Ferrer); Universidad Miguel Hernandez, Sant Joan d´Alacant, Spain (J. Manzanares)
Figure 1. . . Immunoblots of brain extracts from hemizygous 113LBoPrP-Tg037+/− 113LBoPrP-Tg009+/− mouse lines compared with those of cow brain extract and BoPrP-Tg110 mouse brain extract. Brain homogenates were analyzed by Western blotting with monoclonal antibody 2A11 (30). Lane 1, 113LBoPrPTg-009; lane 2, 113LBoPrPTg-037; lane 3, Cow; lane 4, BoPrPTg-110. Equivalent amounts of total protein were loaded into each lane. 113L, leucine substitution at codon 113; BoPrP, bovine prion protein. Values on the right are molecular masses in kilodaltons.
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