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Volume 17, Number 12—December 2011
Letter

Ranavirosis in Invasive Bullfrogs, Belgium

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To the Editor: Massive global declines in amphibians have been attributed to various causes, including infectious diseases such as chytridiomycosis and ranavirosis. Chytridiomycosis and ranaviral disease are international notifiable diseases because they have been listed by the World Organisation for Animal Health in its Animal Health Code.

Ranavirosis is caused by icosahedral cytoplasmic DNA viruses that belong to the family Iridoviridae, in particular by 4 species of Ranavirus: Frog Virus 3 (FV3), Bohle iridovirus, Ambystoma tigrinum virus, and a possible species Rana catesbeiana virus Z. In Europe, FV3 has been identified in several outbreaks of ranavirosis, characterized by mass deaths, notably in green frogs (Pelophylax sp.) in Denmark, Croatia, and the Netherlands (1,2); Rana temporaria and Bufo bufo in the United Kingdom (3,4); and Alytes obstetricans and Ichthyosaura alpestris in Spain (5). The invasive exotic bullfrog (Lithobates catesbeianus) has been introduced in several European countries and has established large breeding populations in France, Italy, Germany, Greece, and Belgium (6).

In addition to their direct effect on native amphibians through competition and predation, bullfrogs are thought to be carriers of chytridiomycosis (7,8) and, possibly, ranaviruses. Although mass deaths of L. catesbeianus tadpoles has been reported in aquaculture facilities, L. catesbeianus tadpoles are generally considered a subclinical reservoir of ranaviruses in the United States (9).

To assess the role of bullfrogs as carriers of ranaviruses in Europe, we collected 400 clinically healthy tadpoles of L. catesbeianus from 3 invasive bullfrog populations at Hoogstraten, Belgium (51°47′Ν, 4°75′Ε) during May–June 2010. All larvae were euthanized as part of an invasive species eradication project and stored at –20°C until further use. At necropsy, liver tissues were collected, and DNA was extracted by using the Genomic DNA Mini Kit (BIOLINE, London, UK). PCR to detect ranavirus was performed as described by Mao et al. (10).

Three samples showed positive results with this PCR. These samples were sequenced by using primers M4 and M5 described by Mao et al. (10) and blasted in GenBank. A 100% homology with the common midwife toad (A. obstetricans) ranavirus partial major capsid protein gene (GenBank accession no. FM213466.1) was found (5). Despite the low prevalence of Ranavirus infection (0.75%) in the bullfrog tadpoles examined, this study shows that invasive bullfrogs, a known reservoir of chytridiomycosis, are also a likely carrier of ranaviral disease in Europe.

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Acknowledgment

This study was partly performed in the framework of European Union Interreg IVA project IVA-VLANED-2.31 “Invasieve exoten in Vlaanderen en Zuid-Nederland–INVEXO.”

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Mojdeh Sharifian-Fard, Frank Pasmans, Connie Adriaensen, Sander Devisscher, Tim Adriaens, Gerald Louette, and An MartelComments to Author 
Author affiliations: Ghent University, Merelbeke, Belgium (M. Sharifian-Fard, F. Pasmans, C. Adriaensen, A. Martel); Research Institute for Nature and Forest, Brussels, Belgium (S. Devisscher, T. Adriaens, G. Louette)

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References

  1. Ariel  E, Kielgast  J, Svart  HE, Larsen  K, Tapiovaara  H, Jensen  BB, Ranavirus in wild edible frogs Pelophylax kl. esculentus in Denmark. Dis Aquat Organ. 2009;85:714. DOIPubMedGoogle Scholar
  2. Fijan  N, Matasin  Z, Petrinec  Z, Valpotiç  I, Zwillenberg  LO. Isolation of an iridovirus-like agent from the green frog (Rana esculenta L.). Vet Arch Zagreb. 1991;3:1518.
  3. Cunningham  AA, Langton  TES, Bennet  PM, Lewin  JF, Drury  SEN, Gough  RE, Pathological and microbiological findings from incidents of unusual mortality of the common frog (Rana temporaria). Philos Trans R Soc Lond B Biol Sci. 1996;351:153957. DOIPubMedGoogle Scholar
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Cite This Article

DOI: 10.3201/eid1712.110236

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An Martel, Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Belgium

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Page created: November 18, 2011
Page updated: November 18, 2011
Page reviewed: November 18, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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