Volume 20, Number 2—February 2014
Dispatch
Molecular Detection of Diphyllobothrium nihonkaiense in Humans, China
Shaohong Chen1, Lin Ai, Yongnian Zhang, Jiaxu Chen, Weizhe Zhang1, Yihong Li, Maki Muto1, Yasuyuki Morishima, Hiromu Sugiyama, Xuenian Xu1, Xiaonong Zhou1, and Hiroshi Yamasaki1
Table
Cases of infection with Diphyllobothrium species in humans reported in mainland China, 1927–2012*
| Case no. | Residence/place of eating fish, if different | Proglottids expelled | Suspected source of infection | Proglottid specimen fixative | Morphologic identification | Molecular identification | GenBank accession no. for cox1 gene | Reference |
|---|---|---|---|---|---|---|---|---|
| 1–4 | Heilongjiang | NA | NA | NA | D. latum | NT | NA | (11) |
| 5 | Beijing | NA | NA | NA | D. latum | NT | NA | (11) |
| 6 | Shanghai | NA | NA | NA | D. latum | NT | NA | (11) |
| 7 | Beijing (returned from United States) | NA | NA | NA | D. latum | NT | NA | (11) |
| 8 | Guangzhou (returned from Argentina) | NA | NA | NA | D. latum | NT | NA | (11) |
| 9 | Heilongjiang | NA | Raw fish | NA | D. latum | NT | NA | (11) |
| 10 | Jilin | NA | Raw fish | NA | D. latum | NT | NA | (11) |
| 11 |
Fujian (lived in Yokohama, Japan, until 1996) |
2003 Jan |
Plecoglossus altivelis |
NA |
D. latum |
NT |
NA |
(12) |
| 12 | Heilongjiang | 2009 Jan | Raw fish | 10% formalin | D. latum | D. nihonkaiense | AB684625 | (13) and this study |
| 13 | Heilongjiang | NA | Salmon | NA | D. latum | D. latum | NA | (14) |
| 14 | Jilin | NA | Salmon | NA | D. latum | D. latum | NA | (14) |
| 15 | Shanghai, 2008-2011/Japan, China | 2011 Dec | Raw sea and freshwater fish | NA | D. latum | D. latum | NA | (15) |
| 16 | Shanghai/Japan (returned from Japan in June 2008; ate raw salmon in Shanghai) | 2008 Oct | Raw salmon | 10% formalin | D. latum | D. nihonkaiense | AB684624 | This study |
| 17 | Shanghai | 2011 Sep | Raw salmon | 70% ethanol | D. latum | D. nihonkaiense | AB684621 | This study |
| 18 | Shanghai. Ate raw salmon in April 2011 | 2011 Jun | Raw salmon | 70% ethanol | D. latum | D. nihonkaiense | AB684622 | This study |
| 19 | Shanghai. Ate raw salmon in 2011 | 2011 Jul | Raw salmon | 70% ethanol | D. latum | D. nihonkaiense | AB684623 | This study |
*Identification of Diphyllobothrium spp. was performed by morphologic identification alone in cases 1–11; organisms in each case were identified as D. latum. Cases 12–19 were assessed by morphologic and molecular identification; morphologic identification of all specimens was D. latum. Molecular identification varied from morphologic findings in 5 of 8 tested specimens. NA, not available; NT, not tested.
1These authors contributed equally to this article.
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