Volume 20, Number 2—February 2014
Dispatch
Molecular Detection of Diphyllobothrium nihonkaiense in Humans, China
Table
Cases of infection with Diphyllobothrium species in humans reported in mainland China, 1927–2012*
Case no. | Residence/place of eating fish, if different | Proglottids expelled | Suspected source of infection | Proglottid specimen fixative | Morphologic identification | Molecular identification | GenBank accession no. for cox1 gene | Reference |
---|---|---|---|---|---|---|---|---|
1–4 | Heilongjiang | NA | NA | NA | D. latum | NT | NA | (11) |
5 | Beijing | NA | NA | NA | D. latum | NT | NA | (11) |
6 | Shanghai | NA | NA | NA | D. latum | NT | NA | (11) |
7 | Beijing (returned from United States) | NA | NA | NA | D. latum | NT | NA | (11) |
8 | Guangzhou (returned from Argentina) | NA | NA | NA | D. latum | NT | NA | (11) |
9 | Heilongjiang | NA | Raw fish | NA | D. latum | NT | NA | (11) |
10 | Jilin | NA | Raw fish | NA | D. latum | NT | NA | (11) |
11 |
Fujian (lived in Yokohama, Japan, until 1996) |
2003 Jan |
Plecoglossus altivelis |
NA |
D. latum |
NT |
NA |
(12) |
12 | Heilongjiang | 2009 Jan | Raw fish | 10% formalin | D. latum | D. nihonkaiense | AB684625 | (13) and this study |
13 | Heilongjiang | NA | Salmon | NA | D. latum | D. latum | NA | (14) |
14 | Jilin | NA | Salmon | NA | D. latum | D. latum | NA | (14) |
15 | Shanghai, 2008-2011/Japan, China | 2011 Dec | Raw sea and freshwater fish | NA | D. latum | D. latum | NA | (15) |
16 | Shanghai/Japan (returned from Japan in June 2008; ate raw salmon in Shanghai) | 2008 Oct | Raw salmon | 10% formalin | D. latum | D. nihonkaiense | AB684624 | This study |
17 | Shanghai | 2011 Sep | Raw salmon | 70% ethanol | D. latum | D. nihonkaiense | AB684621 | This study |
18 | Shanghai. Ate raw salmon in April 2011 | 2011 Jun | Raw salmon | 70% ethanol | D. latum | D. nihonkaiense | AB684622 | This study |
19 | Shanghai. Ate raw salmon in 2011 | 2011 Jul | Raw salmon | 70% ethanol | D. latum | D. nihonkaiense | AB684623 | This study |
*Identification of Diphyllobothrium spp. was performed by morphologic identification alone in cases 1–11; organisms in each case were identified as D. latum. Cases 12–19 were assessed by morphologic and molecular identification; morphologic identification of all specimens was D. latum. Molecular identification varied from morphologic findings in 5 of 8 tested specimens. NA, not available; NT, not tested.
1These authors contributed equally to this article.