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Volume 20, Number 4—April 2014
Dispatch

Invasive Salmonella enterica Serotype Typhimurium Infections, Democratic Republic of the Congo, 2007–2011

Benedikt Ley, Simon Le Hello, Octavie Lunguya, Veerle Lejon, Jean-Jacques Muyembe, François-Xavier WeillComments to Author , and Jan Jacobs
Author affiliations: Institute of Tropical Medicine, Antwerp, Belgium (B. Ley, J. Jacobs); Institut Pasteur, Paris, France (S. Le Hello, F.-X. Weill); Institut National de Recherche Biomédicale, Kinshasa, Democratic Republic of the Congo (O. Lunguya, J.-J. Muyembe); Institut de Recherche pour le Développement, Montpellier, France (V. Lejon)

Main Article

Figure 2

Representative CRISPOL profiles of Salmonella enterica serotype Typhimurium isolates studied. CRISPOL is a recently developed high-throughput assay based on clustered regularly interspaced short palindromic repeats (CRISPR) polymorphisms. Black squares indicate presence of the CRISPR spacer, detected by the corresponding probe; white squares indicates absence of the spacer. The dendrogram was generated by using BioNumerics version 6.6 software (Applied Maths, Sint-Martens-Latem, Belgium) as desc

Figure 2. . Representative CRISPOL profiles of Salmonella enterica serotype Typhimurium isolates studied. CRISPOL is a recently developed high-throughput assay based on clustered regularly interspaced short palindromic repeats (CRISPR) polymorphisms. Black squares indicate presence of the CRISPR spacer, detected by the corresponding probe; white squares indicates absence of the spacer. The dendrogram was generated by using BioNumerics version 6.6 software (Applied Maths, Sint-Martens-Latem, Belgium) as described (5). The CRISPOL types (CTs) detected among the 180 isolates from the Democratic Republic of Congo (DRC) are labeled as DRC in the Source column. Six common CTs of the Pasteur Institute CRISPOL database (labeled as reference) are also shown. These CTs are from strains of serotype Typhimurium 02–1800 (CT34, DT120), 02–5270 (CT21, DT104), LT2 (CT41, DT4), 02–2561 (CT46, DT12), 02–1749 (CT7, DT14) or its monophasic variant of antigenic formula 1,4,[5],12:i:-, 07–1777 (CT1, DT193). For each distinct CT, the numbers of corresponding isolates, their sequence types (STs), and their antimicrobial drug susceptibility testing (AST) data are indicated. For the ST and AST columns, the numbers in parentheses refer to the number (>2) of tested isolates with such result. AST data are shown only for DRC isolates. The resistance types were as follows: R1, ASKTNGSulTmpC; R2, ASKTNGSulTmpCTe; R3, AC; R4, ASSulTmp; R5, ASSulTmpC; R6, ASSulTmpCNal; R7, ASSulTmpCTe; R8, ASulTmpC; R9, SSulTmpC; R10, ACroCazSKTNGSulTmpCTeAzi; and R11, ACroSKTNGSulTmpCTeNaAzi. Abbreviations used in the descriptions of resistance types are as follows: A, amoxicillin; Cro, ceftriaxone; Caz; ceftazidime; S, streptomycin; K, kanamycin; T, tobramycin; N, netilmicin; G, gentamicin; Sul, sulfamethoxazole; Tmp, trimethoprim; C, chloramphenicol; Te, tetracycline; Nal, nalidixic acid; Azi, azithromycin.

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