Volume 7, Number 4—August 2001
THEME ISSUE
West Nile Virus
West Nile Virus
Isolation and Characterization of West Nile Virus from the Blood of Viremic Patients During the 2000 Outbreak in Israel
Musa Hindiyeh*†, Lester M. Shulman*, Ella Mendelson*
, Lea Weiss*, Zehava Grossman*, and Hanna Bin*
, Lea Weiss*, Zehava Grossman*, and Hanna Bin*
Table
Analysis of West Nile patients' isolates, Israel, 2000 outbreak
| Patients | Virus isolate | RT-PCR acute-phase serum | Tissue culture acute-phase serum(CPE+IFA) | RT-PCR infected cell culture | RT-PCR & tissue culture convalescent-phase serum |
|---|---|---|---|---|---|
| 1 | WN-0043 | (-) | (+) | (+) | ND |
| 2 | WN-0233 | (-) | (+) | (+) | ND |
| 3 | WN-0247 | (+) | (+) | (+) | (-) |
| 4 | WN-0304 | (+) | (+) | (+) | (-) |
+, positive result; -, negative result; ND, not done; RT-PCR, reverse transcription-polymerase chain reaction; CPE, cytopathic effect; IFA, indirect immunofluorescence assay.
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