Volume 7, Number 4—August 2001
THEME ISSUE
West Nile Virus
West Nile Virus
Comparative West Nile Virus Detection in Organs of Naturally Infected American Crows (Corvus brachyrhynchos)
Nicholas A. Panella*
, Amy J. Kerst*, Robert S. Lanciotti*, Patricia Bryant†, Bruce Wolf†, and Nicholas Komar*
, Amy J. Kerst*, Robert S. Lanciotti*, Patricia Bryant†, Bruce Wolf†, and Nicholas Komar*
Table
Amount of virus detected by Vero plaque assay and TaqMan reverse transcriptase polymerase chain reaction in American crow organs
| Crow number | Heart | Kidney | Liver | Lung | Spleen | Brain |
|---|---|---|---|---|---|---|
| NJN 5 | +++a/3.40E+03b | +++/5.90E+04 | ++ /8.48E+04 | +++/2.42E+04 | +++/4.09E+03 | +++/5.41E+03 |
| NJN 6 | ++/2.36E+03 | ++/1.12E+04 | -c/1.10E+02 | -/4.61E+02 | +/2.02E+02 | ++/3.12E+02 |
| NJN 7 | ++/9.57E+03 | ++/5.52E+03 | -/7.61E+01 | +/3.46E+03 | +/1.26E+02 | ++/1.76E+03 |
| NJN 8 | +++/2.31E+05 | +++/5.41E+04 | +++/5.61E+05 | +++/3.20E+04 | +++/4.08E+04 | +++/6.18E+04 |
| NJN 9 | +++/2.94E+04 | +++/1.96E+05 | +/2.15E+05 | +++/4.36E+05 | +++/1.17E+05 | +++/1.78E+05 |
| NJN 11 | +++/3.62E+04 | +++/1.06E+04 | +++/1.06E+03 | +++/1.31E+04 | +++/1.07E+03 | +++/2.15E+04 |
| NJN 13 | NT/NT | +/2.54E+02 | -/1.50E+00 | -/1.68E+02 | -/- | +/9.28E+01 |
| NJN 29 | -/- | -/- | -/- | -/- | -/- | ++/6.67E+00 |
| NJN 30 | -/- | -/- | -/- | -/- | -/- | -/- |
| NJN 33 | -/- | -/- | -/- | -/- | -/- | -/ 3.24E+00 |
| NJN 37 | -/- | -/- | -/- | -/- | -/- | -/ 2.10E-01 |
| NJN 40 | -/- | -/- | -/- | -/- | -/- | -/- |
| NJN 41 | -/- | -/- | -/- | -/- | -/- | -/- |
| NJN 43 | -/- | -/- | -/- | -/- | -/- | -/- |
| NJN 44 | -/- | -/- | -/- | -/- | -/- | -/- |
| NJN 51 | -/- | -/- | -/- | -/- | -/- | -/- |
| NJN 57 | -/- | -/- | -/- | -/- | -/- | -/- |
| NJN 62 | -/- | -/- | -/- | -/- | -/- | -/- |
| NJN 75 | -/- | -/- | -/- | -/- | -/- | -/- |
| NJN 95 | -/- | -/- | -/- | -/- | -/- | -/- |
aVero cell plaque assay: +++ = >100 PFU/200 µL, ++ = 10-100 PFU/200 µL, + = <10 PFU/200 µL.
bTaqMan RT-PCR assay: PFU equivalents/5 µL.
cNegative.
NT = Not tested.
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