Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 14, Number 8—August 2008
Dispatch

Genotyping Rickettsia prowazekii Isolates

Yong Zhu*, Aaron Medina-Sanchez*, Donald H. Bouyer*, David H. Walker*, and Li Zhao*Comments to Author 
Author affiliations: *University of Texas Medical Branch, Galveston, Texas, USA;

Main Article

Table 1

Primers for 6 loci of Rickettsia prowazekii genomic DNA sequences

Target sequence Sequences of forward primer/reverse primer (5′ → 3′) PCR product size, bp Reference
rp028 TTGATATAGGTTGCGGAGTCGGTGTTA/TCATTGATGGCTTGTAGTTTTTCTGCT 682 (9)
rp181 ATTATGCAAATAATGCAG/GCATCGGATAAGTTAGTTCA 390 This study
rp195 TTTATTGGGGATTTACCTTT/CAAGTGTTAGATAGCTTGCT 384 This study
rp272/rp273 TCTTGCGATACAGTAAGCAC/TATTCGCTCCTTACCAGTTA 612 This study
rp308/rp309 TTAACAGAAGTAATAATAATTG/AGCAATAGAATTTGATAAGCA 369 This study
rp691/rp692 AGAAATTTGTATTGCATTTTTATG/GCTCTAGAAGCTATTGCTGA 447 This study

Main Article

References
  1. Raoult  D, Woodward  T, Dumler  JS. The history of epidemic typhus. Infect Dis Clin North Am. 2004;18:12740. DOIPubMedGoogle Scholar
  2. Raoult  D, Roux  V, Ndihokubwayo  JB, Bise  G, Baudon  D, Marte  G, Jail fever (epidemic typhus) outbreak in Burundi. Emerg Infect Dis. 1997;3:35760.PubMedGoogle Scholar
  3. Bozeman  FM, Sonenshine  DE, Williams  MS, Chadwick  DP, Lauer  DM, Elisberg  BL. Experimental infection of ectoparasitic arthropods with Rickettsia prowazekii (GvF-16 strain) and transmission to flying squirrels. Am J Trop Med Hyg. 1981;30:25363.PubMedGoogle Scholar
  4. Duma  RJ, Sonenshine  DE, Bozeman  FM, Veazey  JM Jr, Elisberg  BL, Chadwick  DP, Epidemic typhus in the United States associated with flying squirrels. JAMA. 1981;245:231823. DOIPubMedGoogle Scholar
  5. Andersson  SG, Zomorodipour  A, Andersson  JO, Sicheritz-Ponten  T, Alsmark  UC, Podowski  RM, The genome sequence of Rickettsia prowazekii and the origin of mitochondria. Nature. 1998;396:13340. DOIPubMedGoogle Scholar
  6. Medina-Sanchez  A, Bouyer  DH, Cantara-Rodriguez  V, Mafra  C, Zavala-Castro  J, Whitworth  T, Detection of a typhus group Rickettsia in Amblyomma ticks in the state of Nuevo Leon, Mexico. Ann N Y Acad Sci. 2005;1063:32732. DOIPubMedGoogle Scholar
  7. Balayera  NM, Nikolskaya  VN. Enhanced virulence of the vaccine strain E of Rickettsia prowazekii on passaging in white mice and guinea pigs. Acta Virol. 1972;16:802.PubMedGoogle Scholar
  8. Reiss-Gutfreund  RJ. The isolation of Rickettsia prowazeki and mooseri from unusual sources. Am J Trop Med Hyg. 1966;15:9439.PubMedGoogle Scholar
  9. Zhang  JZ, Hao  JF, Walker  DH, Yu  XJ. A mutation inactivating the methyltransferase gene in avirulent Madrid E strain of Rickettsia prowazekii reverted to wild type in the virulent revertant strain. Vaccine. 2006;24:231723. DOIPubMedGoogle Scholar
  10. Ching  WM, Carl  M, Dasch  GA. Mapping of monoclonal antibody binding sites on CNBr fragments of the S-layer protein antigens of Rickettsia typhi and Rickettsia prowazekii. Mol Immunol. 1992;29:95105. DOIPubMedGoogle Scholar
  11. Ching  WM, Wang  H, Davis  J, Dasch  GA. Amino acid analysis and multiple methylation of lysine residues in the surface protein antigen of Rickettsia prowazekii. In: Angeletti RH, editor, Techniques in protein chemistry, Vol. IV. San Diego: Academic Press. 1993:307–14.
  12. Zhu  Y, Fournier  PE, Ogata  H, Raoult  D. Multispacer typing of Rickettsia prowazekii enabling epidemiological studies of epidemic typhus. J Clin Microbiol. 2005;43:470812. DOIPubMedGoogle Scholar
  13. Ge  H, Chuang  YY, Zhao  S, Tong  M, Tsai  MH, Temenak  JJ, Comparative genomics of Rickettsia prowazekii Madrid E and Breinl strains. J Bacteriol. 2004;186:55665. DOIPubMedGoogle Scholar
  14. Ge  H, Tong  M, Jiang  J, Dasch  GA, Richards  AL. Genotypic comparison of five isolates of Rickettsia prowazekii by multilocus sequence typing. FEMS Microbiol Lett. 2007;271:1127. DOIPubMedGoogle Scholar

Main Article

Page created: July 13, 2010
Page updated: July 13, 2010
Page reviewed: July 13, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external