Volume 14, Number 8—August 2008
Dispatch
Genotyping Rickettsia prowazekii Isolates
Yong Zhu*, Aaron Medina-Sanchez*, Donald H. Bouyer*, David H. Walker*, and Li Zhao*
Table 2
Genotypes of Rickettsia prowazekii strains determined by nucleotide mutation in multiple loci
| Strain | rp028* |
rp181 |
rp195 |
rp272–rp273 |
rp308–rp309 |
rp691–rp692 |
GT | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 268† | 286 | 480 | 732 | 713–714 | 140 | 1529 | 52–53 | 306 | 1306–1307 | 1415 | |||||||
| GvV-250 | T | G | C | – | – | TACTTCAAGCTCATTTCG | C | AA | GTCATTATCGTAT | TT | G | 1 | |||||
| GvF-16 | T | G | T | – | – | TACTTCAAGCTCATTTCG | C | AA | GTCATTATCGTAT | TT | G | 2 | |||||
| Breinl | T | A | C | – | – | TACTTCAAGCTCATTTCG | G | – | GTCATTATCGTAT | – | – | 3 | |||||
| Cairo | T | A | C | – | G | TACTTCAAGCTCATTTCG | G | A | GTCATTATCGTAT | – | – | 4 | |||||
| ZRS | G | A | C | – | G | – | G | AA | – | TT | – | 5 | |||||
| Addis Ababa | G | A | C | – | G | – | G | AA | – | TT | – | 5 | |||||
| Madrid E | G | A | C | A | GG | – | G | AA | – | TT | – | 6 | |||||
| Evir | G | A | C | – | GG | – | G | AA | – | TT | – | 7 | |||||
*Gene names or intergenic spacers between genes.
†Positions of nucleotides with mutation, which were counted from the first nucleotide of the coding sequence or the first nucleotide after the stop codon in the case of intergenic spacers; –, deletion of nucleotides, in which the number of nucleotides deleted equals the nucleotides in the same column for the corresponding strains that do not have the deletion. For example, in rp181, the GvV-250 strain has 1 deleted nucleotide when compared with the Cairo strain, but it has deleted 2 nucleotides when compared with the E strain.
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