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Volume 15, Number 8—August 2009
Letter

Human Rhinovirus Group C in Hospitalized Children, Singapore

Boon-Huan TanComments to Author , Liat-Hui Loo, Elizabeth Ai-Sim Lim, Shirley Lay-Kheng Seah, Raymond T.P. Lin, Nancy W.S. Tee, and Richard J. Sugrue
Author affiliations: DSO National Laboratories, Singapore (B.-H. Tan, E.A.-S. Lim, S.L.-K. Seah); Nanyang Technological University, Singapore (L.-H. Loo, R.J. Sugrue); Kandang Kerbau Women’s and Children’s Hospital, Singapore (L.-H. Loo, N.W.S. Tee); National University Hospital, Singapore (R.T.P. Lin)

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Figure

Phylogenetic analysis of human rhinoviruses (HRVs) from Singapore based on nucleotide sequences of the 5′ noncoding region. The tree was constructed by using the neighbor-joining method with 1,000 bootstrapped replicates generated by MEGA version 4 software (9). Sequences (GenBank accession nos. FJ645828–FJ645771) of viruses from Singapore (SIN) are indicated, where the 2 numbers represent the year the specimen was collected, and NTU (Nanyang Technological University) followed by 3 numbers represents the specimen number. Representative strains of HRV-C are indicated by squares, HRV-B by circles, and HRV-A by triangles. RV indicates rhinovirus strains, followed by the serotype no. These sequences were obtained from the report by Lee et al. (3). Boldface indicates a cluster of 10 HRV-A strains that diverged from reference HRV-A strains. Scale bar indicates nucleotide substitutions per site.

Figure. Phylogenetic analysis of human rhinoviruses (HRVs) from Singapore based on nucleotide sequences of the 5′ noncoding region. The tree was constructed by using the neighbor-joining method with 1,000 bootstrapped replicates generated by MEGA version 4 software (9). Sequences (GenBank accession nos. FJ645828–FJ645771) of viruses from Singapore (SIN) are indicated, where the 2 numbers represent the year the specimen was collected, and NTU (Nanyang Technological University) followed by 3 numbers represents the specimen number. Representative strains of HRV-C are indicated by squares, HRV-B by circles, and HRV-A by triangles. RV indicates rhinovirus strains, followed by the serotype no. These sequences were obtained from the report by Lee et al. (3). Boldface indicates a cluster of 10 HRV-A strains that diverged from reference HRV-A strains. Scale bar indicates nucleotide substitutions per site.

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References
  1. Lamson  D, Renwick  N, Kapoor  V, Liu  Z, Palacios  G, Ju  J, MassTag polymerase-chain-reaction detection of respiratory pathogens, including a new rhinovirus genotype, that caused influenza-like illness in New York State during 2004–2005. J Infect Dis. 2006;194:1398–402.DOIGoogle Scholar
  2. Lau  SK, Yip  CC, Tsoi  HW, Lee  RA, So  LY, Lau  YL, Clinical features and complete genome characterization of a distinct human rhinovirus (HRV) genetic cluster, probably representing a previously undetected HRV species, HRV-C, associated with acute respiratory illness in children. J Clin Microbiol. 2007;45:3655–64.DOIGoogle Scholar
  3. Lee  WM, Kiesner  C, Pappas  T, Lee  I, Grindle  K, Jartti  T, A diverse group of previously unrecognized human rhinoviruses are common causes of respiratory illnesses in infants. PLoS One. 2007;2:e966.DOIGoogle Scholar
  4. McErlean  P, Shackelton  LA, Lambert  SB, Nissen  MD, Sloots  TP, Mackay  IM. Characterisation of a newly identified human rhinovirus, HRV-QPM, discovered in infants with bronchiolitis. J Clin Virol. 2007;39:67–75.DOIGoogle Scholar
  5. Xiang  Z, Gonzalez  R, Xie  Z, Xiao  Y, Chen  L, Li  Y, Human rhinovirus group C infection in children with lower respiratory tract infection. Emerg Infect Dis. 2008;14:1665–7.DOIGoogle Scholar
  6. Loo  LH, Tan  BH, Ng  LM, Tee  NW, Lin  RT, Sugrue  RJ. Human metapneumovirus in children, Singapore. Emerg Infect Dis. 2007;13:1396–8.
  7. Tan  BH, Lim  EA, Seah  SG, Loo  LH, Tee  NW, Lin  RT, The incidence of human bocavirus infection among children admitted to hospital in Singapore. J Med Virol. 2009;81:82–9.DOIGoogle Scholar
  8. Hayden  FG, Turner  RB, Gwaltney  JM, Chi-Burris  K, Gersten  M, Hsyu  P, Phase II, randomized, double-blind, placebo-controlled studies of ruprintrivir nasal spray 2-percent suspension for prevention and treatment of experimentally induced rhinovirus colds in healthy volunteers. Antimicrob Agents Chemother. 2003;47:3907–16.DOIGoogle Scholar
  9. Tamura  K, Dudley  J, Nei  M, Kumar  S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007;24:1596–9.DOIGoogle Scholar
  10. Miller  EK, Edwards  KM, Weinberg  GA, Iwane  MK, Griffin  MR, Hall  CB, New Vaccine Surveillance Network. A novel group of rhinoviruses is associated with asthma hospitalizations. J Allergy Clin Immunol. 2009;123:98–104.DOIGoogle Scholar

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