Volume 16, Number 3—March 2010
Letter
Yersinia Species Isolated from Bats, Germany
Table
Strain typing results of Yersinia spp. isolates from free-ranging bats, Germany*
Method | Y. pseudotuberculosis† | Y. enterocolitica‡ |
---|---|---|
Api 20E profile§ | 1 0 1 4 1 1 2 1 7 | 1 1 5 4 7 2 3 1 7 |
Micronaut E,¶ % probability | 100% Y. pseudotuberculosis | 99.77% Y. enterocolitica |
16S rRNA gene analysis, % similarity to sequences in GenBank | 100% (1,058 bp) to Y. pseudotuberculosis YPIII# | 100% (985 bp) to Y. enterocolitica strain 280** |
Serologic characterization by agglutination test | Serogroup 1, biovar 5 | Serotype O:6, biovar 1A |
Multilocus sequence typing†† | 1 2 1 2 5 2 1 | Not determined |
*Y. pseudotuberculosis obtained from lung, heart, kidney, spleen, liver, and intestine; Y. enterocolitica obtained from spleen and intestine. For Y. pseudotuberculosis spleen, liver, and intestine samples, isolates were obtained in mixed culture accompanied by colonies of 1–2 nonspecific bacteria. For all remaining tissues, Y. pseudotuberculosis and Y. enterocolitica were isolated in pure culture.
†GenBank accession no. FN561631.
‡GenBank accession no. FN561632.
§bioMérieux, Nürtingen, Germany.
¶Merlin Diagnostik GmbH, Bornheim-Hersel, Germany.
#GenBank accession no. CP000950.
**GenBank accession no. FJ641888.
††Of gene loci adk, argA, aroA, glnA, thrA, tmk, trpE.