Volume 10, Number 12—December 2004
Dispatch
SARS Molecular Detection External Quality Assurance
Table 2
Factors influencing the performance of laboratoriesa
| Possible technical influence factors | No. of laboratories | Positive influence on sensitivity p value |
|---|---|---|
| Qiagen viral RNA extraction kit | 38 | 0.9 |
| Roche MagnaPure/HighPure extraction kit | 7 | 0.2 |
| Silica particle-based extraction method (Boom) | 9 | 0.9 |
| Primers originally developed in own laboratory | 16 | 0.5 |
| Any nested PCR assay | 25 | 0.9 |
| Any real-time PCR assay | 37 | 0.7 |
| Any commercial test kit | 14 | 0.03 |
aAnalysis of Variance (ANOVA) by factor, eliminating the influence of other factors; PCR, polymerase chain reaction.
1University Vienna, Vienna, Austria; University Hospital Leuven, Leuven, Belgium; Statens Serum Institut, Copenhagen, Denmark; Health Protection Agency, London and Salibury. England; University of Helsinki, Helsinki, Finland; Institut Pasteur, Paris, France; Bernhard Nocht Institut, Hamburg, Germany; Philipps Universität, Marburg, Germany; Robert Koch-Institut, Berlin, Germany; University Frankfurt, Frankfurt, Germany; M & LAT, Berlin, Germany; Artus GmbH, Hamburg, Germany; Euroimmun, Lübeck, Germany; Aristotelian University, Thessaloniki, Greece; University of Athens, Athens, Greece;National Center for Epidemiology, Budapest, Hungary; University Hospital Reykjavik, Reykjavik, Iceland; Chaim Sheba Medical Center, Tel Hashomer, Israel; Army Medical and Veterinary Research Center, Rome, Italy; Istituto Nazionale Malattie Infettive, Rome, Italy; Istituto Superiore di Sanità, Rome, Italy; Erasmus MC, Rotterdam, the Netherlands; RIVM, Bilthoven, the Netherlands; Leiden University Medical Center, Leiden, the Netherlands; Norwegian Institute of Public Health, Oslo, Norway; National Institute of Health, Warsaw, Poland; National Institute of Health, Lisboa, Portugal; Central Research Institute for Epidemiology, Moscow, Russia; University of Ljubljana, Ljubljana, Slovenia; Instituto de Salud Carlos III, Madrid, Spain; Swedish Institute for Infectious Disease Control, Solna, Sweden; IKMI, St. Gallen, Switzerland; Hôpital Central Universitare Geneve, Geneva; Switzerland; Institut Pasteur de Dakar, Dakar, Senegal; National Institute of Health, Buenos Aires, Argentina; Fundacao Oswaldo Cruz, Rio de Janeiro, Brazil; Canadian Center for Human & Animal Health, Winipeg, Canada; Sunnybrook & Women's College Health Sciences Centre, Toronto, Canada; Clinical and Central Public Health Laboratory, Etobicoke, Canada; National Institute of Health, Nunoa Santiago, Chili; Instituto Conmemorativa Gorgas, Panama, Panama; CDC, Atlanta, Georgia, USA; Focus Technologies, Cypress, CA, USA; Governmental Virus Unit, Hong Kong, China; University of Hong Kong, Hong Kong, China; Peking University, Peking, China; CDC Beijing, Beijing, China; NIHRD, Jakarta, Indonesia; National Institute of Infectious Diseases, Tokyo, Japan; National Institute of Health, Seoul, Korea; Singapore General Hospital, Singapore; National Institute of Health, Nonthaburi, Thailand; Westmead Hospital, Westmead, Australia; Victorian Infectious Disease. Laboratory, Melbourne, Australia; LabPlus, Aukland Hospital, Aukland, New Zealand; Institute of Environmental Research, Porirua, New Zealand.