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Volume 10, Number 12—December 2004


Genome Sequence and Attenuating Mutations in West Nile Virus Isolate from Mexico

David W.C. Beasley*Comments to Author , C. Todd Davis*, Jose Estrada-Franco*, Roberto Navarro-Lopez†, Arturo Campomanes-Cortes†, Robert B. Tesh*, Nikos Vasilakis*, and Alan D.T. Barrett*
Author affiliations: *University of Texas Medical Branch, Galveston, Texas, USA; †Comision Mexico-Estados Unidos para la Prevencion de la Fiebre Aftosa y Otras Enfermedades Exoticas de los Animales, Mexico City, Mexico

Main Article

Table 1

Summary of nucleotide and amino acid differences between West Nile virus strains NY99 (AF196835), NY00-grouse3282 (AF404755), and TM171-03a

(amino acid) NY99 NY00 TM171-03
50 A A G
71 A A G
93 C T C
381 C C T
483 C C T
858 C C T
887 (prM-141) T (Ile) T (Ile) C (Thr)
1137 C C T
1285 C T C
1432 (E-156)b T (Ser) T (Ser) C (Pro)
1626 C C T
2328 C C T
2388 C C T
2466 C C T
2607 T T C
2832 T T C
2865 C C T
3111 G G A
4146 A G G
4212 T T A
4564 T C C
4749 C C T
6120 C C T
6138 C T T
6141 C C T
6279 G G A
6426 C T T
6495 G G A
6771 A G G
7015 T C C
7359 C C T
7648 (NS4B-245) A (Ile) A (Ile) G (Val)
7672 C C T
7938 T C C
8067 A G A
8109 C C T
8676 A A G
8811 T C C
8838 T T C
8994 T T C
9378 T T C
9408 T T C
9453 C C T
10317 C C T
10373 (NS5-898) C (Thr) C (Thr) T (Ile)
10393 C T T
10828 T T G
10851 A G G
10989 G G A

aBold text indicates residues at which NY00-grouse 3282 and TM171-03 both differed from NY99.
bStrain TM171-03 had a mixed sequence at this residue. Consensus sequence of early passage material had “C” at nucleotide 1432.

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