Volume 10, Number 4—April 2004
Research
Ixodid and Argasid Tick Species and West Nile Virus
Charles Henderson Lawrie*
, Nathalie Yumari Uzcátegui*, Ernest Andrew Gould*, and Patricia Anne Nuttall*
, Nathalie Yumari Uzcátegui*, Ernest Andrew Gould*, and Patricia Anne Nuttall*
Table 2
Nucleotide sequences of primers used in first round (5′[1] and 3′[2]) and second round (5′[2] and 3′[2]) of nested RT-PCRa
| Primer | Sequence | Position in WNV (NY99)b |
|---|---|---|
| 5′(1) |
CCATATGAATTCCATGAGTGCTATCAATCGGCGGAG |
31 aa upstream (C gene) from start of PrM gene (373) |
| 3′(1) |
CATATGCGGCCGCTTACTAGGTGATTGATCTGTTGTTCTCC |
31 aa downstream (NS1) from end of E gene (2,562) |
| 5′(2) |
CATATGCGGCCGCTTACTACCGGTCCATCCAAGCCTC |
Start of E gene (967) |
| 3′(2) | CCATATAGATCTCGGAGGTCATTCAACTGCCTTGGAATGAGC | 395 aa into E gene (2,152) |
aRT-PCR, reverse transcriptase–polymerase chain reaction; aa, amino acids.
bNucleotide position in relation to complete genome sequence of WNV (NY99) shown in parentheses (accession no. AF19685).
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