Volume 11, Number 2—February 2005
Isolation of Waddlia malaysiensis, A Novel Intracellular Bacterium, from Fruit Bat (Eonycteris spelaea)
|Gene target||Primer sequence|
|16S rRNA (1,526 bp from ref. 20)|
|16S-FOR||5′ AGA GTT TGA TCC TGG 3′|
|16S-REV||5′ TAC CTT GTT ACG ACT T 3′|
|Tm = 55ºC|
|16S rRNA signature sequence (298 bp from ref. 21)|
|16S1GF||5′ CGG CGT GGA TGA GGC AT 3′|
|16S1GR||5′ TCA GTC CCA GTG TTG GC 3′|
|Tm = 51º C|
|16S – 23S rRNA signature sequence (1 kbp from ref. 21)|
|16SF2||5′ CCG CCC GTC ACA TCA TGG 3′|
|23S1GR||5′ TGG CTC ATC ATG CAA AAG GCA 3′|
|Tm = 61º C|
|23S rRNA signature sequence (627 bp: domain I from ref. 21)|
|23S1GR||5′ TGG CTC ATC ATG CAA AAG GCA 3′|
|Tm = 61º C|
|MurA signature sequence (690 bp from ref. 22)|
|murA-for||5′ GTN GGN GCN ACN GAR AA 3'|
|murA-rev||5′ GCC ATN ACR TAN GCR AAN CCN GC 3′|
|Tm = 55º C|
|sctN (331 bp)|
|sctN FOR||5' AGA RGG AAT GAA ACG TTC 3'|
|sctN REV||5' GGC TCR TTC ATA TCA TC 3'|
|Tm = 58º C|
|16S rRNA (1,526 bp)|
|F1 (M13)||5 GTT TTC CCA GTC ACG ACG TTG TA 3′|
|F2||5′ GCT CAC CAA GGC TAA GAC GTC 3′ (277-298)|
|F3||5′ CTA GCT TTG ACC TGA CGC TGA T 3′ (752-774)|
|F4||5′ GAA TCT GCA ACT CGG CTC CAT G 3′ (1323-1345)|
|R1 (M13)||5′ TTG TGA GCG GAT AAC AAT TTC 3′|
|R2||5′ CAT CCT AAA TGC TGG CAA C 3' (392-373)|
|R3||5′ CAC CGC TAC ATG TGG AAT TCC 3′ (843-822)|
|R4||5′ GAT CCT CTC TAG CAC CAT ATC C 3′ (1358-1336)|
*PCR, polymerase chain reaction; Tm, melting temperature.
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