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Volume 11, Number 2—February 2005
Books and Media

DNA Amplification: Current Technologies and Applications

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Vadim V. Demidov; Natalia E. Broude
Horizon Bioscience, Norfolk, UK
IBSN: 0-9545232-9-6
Pages: 335, Price: U.S.$180

DNA amplification is a powerful technique that has had an immense impact on scientific research in the past 2 decades. While polymerase chain reaction (PCR) is still the most popular method, alternative methods of DNA amplification are constantly being developed. In addition, the extraordinary versatility of PCR has led to its use in novel ways that have opened new avenues of research. These novel methods for DNA amplification and the versatility of PCR are highlighted in DNA Amplification: Current Technologies and Applications.

The 17 chapters in this book are divided into 4 sections that focus on enzymes (3 chapters), thermal cycling methods (6 chapters), isothermal methods (6 chapters), and the detection of non-DNA analytes by DNA amplification (2 chapters). Each chapter has a thorough description of methods and highly detailed protocols for applying the technique to at lease 1 specific application. Several excellent chapters describe the uses of Phi29 DNA polymerase and of applications using isothermal rolling circle amplification. A chapter on multiple-displacement amplification details the isothermal amplification of total genomic DNA and should prove extremely useful for amplifying DNA in limited amounts, such as DNA from clinical samples. The final 2 chapters describe use of either real-time PCR or rolling circle amplification to detect and quantify non-DNA analytes, such as serum cytokines, with much greater sensitivity than conventional enzyme-linked immunosorbent assay methods.

This book is not for the novice scientist, as it does not describe basic DNA amplification fundamentals; rather, it is directed at those with a solid background in molecular biology who desire knowledge of cutting-edge applications. Although many of the detailed protocols will not be applicable to certain laboratory situations, the versatility of most of the methods described will allow them to be easily adapted to other studies. Therefore, this book will be a good addition to the library of researchers in molecular biology or to molecular diagnostics laboratories planning to expand their horizon beyond standard PCR amplification techniques.

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Robert Massung*Comments to Author 

Author affiliation: *Centers for Disease Control and Prevention, Atlanta, Georgia, USA

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Cite This Article

DOI: 10.3201/eid1102.041049

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Table of Contents – Volume 11, Number 2—February 2005

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Robert Massung, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30333, USA; fax: 404-639-4436

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Page created: May 09, 2011
Page updated: May 09, 2011
Page reviewed: May 09, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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