Volume 11, Number 7—July 2005
Dispatch
Cervids as Babesiae Hosts, Slovenia
Darja Duh*
, Miroslav Petrovec*, Andrej Bidovec†, and Tatjana Avsic-Zupanc*
, Miroslav Petrovec*, Andrej Bidovec†, and Tatjana Avsic-Zupanc*
Table
Nucleotide sequences and optimum annealing temperatures of the primers designed and used for nested polymerase chain reaction (PCR) and those for amplifying and sequencing the complete babesial 18S rRNA gene
| Primer | Nucleotide sequence (5´→3´) | Annealing temperature (°C) |
|---|---|---|
| BABSr* | CTC CAA TCC CTA GTC GGC A | 60 |
| BOD* | GTT ATT GAC TCT TGT CTT TAA | 53 |
| BDV* | AAT ATT GAC TGA TGT CGA GAT | 53 |
| CRYPTO F | AAC CTG GTT GAT CCT GCC AGT AGT CAT | 60 (6) |
| PIRO A* | AAT ACC CAA TCC TGA CAC AGG G | (7) |
| PIRO B* | TTA AAT ACG AAT GCC CCC AAC | (7) |
| PIRO C | GTT GGG GGC ATT CGT ATT TAA | 60 |
| 1055 F | GGT GGT GCA TGG CCG | 60 |
| 1055 R | AAC GGC CAT GCA CCA C | 60 |
| 1200F | CAG GTC TGT GAT GCC | 60 |
| 1200 R | GGG CAT CAC AGA CCT G | 60 |
| CRYPTO R | GAA TGA TCC TTC CGC AGG TTC ACC TAC | 60 (6) |
*Primers used for nested PCR.
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