Volume 11, Number 7—July 2005
Letter
Pandemic Vibrio parahaemolyticus O3:K6 Spread, France
Table
Strain no. CNRVC (source no.) | Origin | Date of isolation | Source of transmission | Detection of gene or phage sequences by PCR |
|||||||
---|---|---|---|---|---|---|---|---|---|---|---|
R72H | tdh | trh | toxRS | orf8 | Ribotype profile | PFGE profile | AP-PCR profile | ||||
970136 | France (Atlantic coast) | Oct 1997 | Local oysters | + | + | - | + | + | R4† | P-1a | AP-a |
980402 | France (southwest) | Sep 1998 | Shellfish | + | + | - | + | + | R4 | P-1c | AP-a |
990346 | France (Mediterranean coast) | Aug 1999 | – | + | + | - | + | + | R4 | P-1c | AP-a |
030478 | France (Atlantic coast) | Aug 2003 | Local shellfish | + | + | - | + | + | R4 | UT‡ | AP-a |
030479 | France (Atlantic coast) | Aug 2003 | – | + | + | - | + | + | R4 | UT | AP-a |
020468 (AN7410) | Bangladesh | 1998 | + | + | - | + | + | R4 | P-1a | AP-a | |
020469 (AO1851) | Bangladesh | 1999 | + | + | - | + | + | R4 | P-1b | AP-a | |
030085 (AQ4037) | Maldives | 1985 | + | - | + | - | - | Rb-2§ | P-2 | AP-b |
*CNRVC, Centre National de Référence des Vibrions et du Cholera; PCR, polymerase chain reaction; PFGE, pulsed-field gel electrophoresis; AP-PCR, arbitrarily primed PCR.
†R4 ribotype pattern as described previously (5).
‡UT, untypeable: DNA was degraded before PFGE, presumably by DNases.
§According to our pattern designation.
References
- Matsumoto C, Okuda J, Ishibashi M, Iwanaga M, Garg P, Rammamurthy T, Pandemic spread of an O3:K6 clone of Vibrio parahaemolyticus and emergence of related strains evidenced by arbitrarily primed PCR and toxRS sequence analyses. J Clin Microbiol. 2000;38:578–85.PubMedGoogle Scholar
- Nishibuchi M, Kaper JB. Thermostable direct hemolysin gene of Vibrio parahaemolyticus: a virulence gene acquired by a marine bacterium. Infect Immun. 1995;63:2093–9.PubMedGoogle Scholar
- Myers ML, Panicker G, Bej AK. PCR detection of a newly emerged pandemic Vibrio parahaemolyticus O3:K6 pathogen in pure cultures and seeded waters from the Gulf of Mexico. Appl Environ Microbiol. 2003;69:2194–200. DOIPubMedGoogle Scholar
- Robert-Pillot A, Guénolé A, Fournier JM. Usefulness of R72H PCR assay for differentiation between Vibrio parahaemolyticus and Vibrio alginolyticus species: validation by DNA-DNA hybridization. FEMS Microbiol Lett. 2002;215:1–6.PubMedGoogle Scholar
- Bag PK, Nandi S, Bhadra RK, Ramamurthy T, Bhattacharya SK, Nishibuchi M, Clonal diversity among recently emerged strains of Vibrio parahaemolyticus O3:K6 associated with pandemic spread. J Clin Microbiol. 1999;37:2354–7.PubMedGoogle Scholar
- Daniels NA, Ray B, Easton A, Marano N, Kahn E, McShan AL, Emergence of a new Vibrio parahaemolyticus serotype in raw oysters—A prevention quandary. JAMA. 2000;284:1541–5. DOIPubMedGoogle Scholar
- McCarthy SA, Khambaty FM. International dissemination of epidemic Vibrio cholerae by cargo ship ballast and other nonpotable waters. Appl Environ Microbiol. 1994;60:2597–601.PubMedGoogle Scholar
- Mead PS, Slutsker L, Dietz V, McGaig LF, Bresee JS, Shapiro C, Food-related illness and death in the United States. Emerg Infect Dis. 1999;5:607–25. DOIPubMedGoogle Scholar
- Robert-Pillot A, Guénolé A, Lesne J, Delesmont R, Fournier JM, Quilici ML. Occurrence of the tdh and trh genes in Vibrio parahaemolyticus isolates from waters and raw shellfish collected in two French coastal areas and from seafood imported into France. Int J Food Microbiol. 2004;91:319–25. DOIPubMedGoogle Scholar
- Centers for Disease Control and Prevention. Outbreak of Vibrio parahaemolyticus infections associated with eating raw oysters—Pacific Northwest, 1997. MMWR Morb Mortal Wkly Rep. 1998;47:457–62.PubMedGoogle Scholar
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