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Volume 12, Number 11—November 2006

Letter

Real-time PCR for Francisella tularensis Types A and B

Kiersten J. Kugeler*, Ryan Pappert*, Yan Zhou*, and Jeannine M. Petersen*Comments to Author 
Author affiliations: *Centers for Disease Control and Prevention, Fort Collins, Colorado, USA

Main Article

Table A1

Specificity evaluation with DNA from Francisella spp

Organism Sample ID Source Geographic origin Type A assay
(Ct value)* Type B assay
(Ct value)*
F. tularensis subsp.
tularensis (type A)
SchuS4 Human Ohio 15.9 –
WY963418 Human Wyoming 15.9 –
MA002987 Human Massachusetts 18.4 –
CO012364 Cat Colorado 18.3 –
CO013713 Rabbit Colorado 16.5 –
ND000952 Human North Dakota 16.9 –
KS000948 Cat Kansas 16.7 –
OK002731 Human Oklahoma 18.4 –
NC993990 Rabbit North Carolina 16.8 –
AR000028 Human Arkansas 18.8 –
UT983134 Human Utah 16.9 –
NM990295 Rabbit New Mexico 17.2 –
NC973057 Rabbit North Carolina 16.5 –
NC015379 Cat North Carolina 18.6 –
AR982146 Rabbit Arkansas 18.0 –
OK004337 Human Oklahoma 18.4 –
AR011117 Human Arkansas 22.9 –
MO011907 Human Missouri 20.3 –
CA020099
Human
California
18.2
–
F. tularensis subsp.
holarctica (type B)
LVS Rat Russia – 16.0
KY993387 Human Kentucky – 19.7
OR960246 Monkey Oregon – 16.7
CN985979 Human Canada – 17.1
AZ001325 Rat Arizona – 16.2
IL003633 Human Illinois – 18.4
MO011673 Human Missouri – 16.8
KY001708 Human Kentucky – 15.8
OH013029 Prairie dog Ohio – 16.7
UT002098 Human Missouri – 18.2
SP986120 Rabbit Spain – 17.5
IN983055 Rat Indiana – 15.6
CO961243 Vole Colorado – 15.5
CA990837 Human California – 16.5
IN002758 Human Indiana – 15.8
AZ001324 Squirrel Arizona – 15.9
CA993992 Monkey California – 16.9
SP982108 Human Spain – 17.8
NM002642 Human New Mexico – 17.9
JAP5-3-11 Human Japan – 18.6
KO971026 Human Korea – 19.0
F. tularensis subsp.
novicida
GA993548 Human Louisiana – –
GA993549 Human California – –
GA993550 Water Utah – –
UT014992 Human Utah – –
AS020814 Human Australia – –
FX1 Human Texas – –
FX2
Human
Texas
–
–
F. philomiragia
GA012793 Human California – –
GA012794 Human Colorado – –
GA012795 Human New York – –
GA012796 Human California – –
GA012797 Human Pennsylvania – –
GA012799 Human Connecticut – –
GA012800 Human Connecticut – –
GA012801 Human New York – –
GA012802 Human California – –
GA012803 Human New Mexico – –
GA012804 Human Virginia – –
GA012806 Human Massachusetts – –
GA012810 Water Utah – –
GA012811 Water Utah – –
ATCC 25015
Muskrat
Utah
–
–
Francisella-like
tick endosymbionts†
2040372 Tick California – –
2040460 Tick California – –
MV2
Tick
Massachusetts
–
–
Francisella-like
bacteria‡
005 Soil Texas – –
013 Soil Texas – –
015 Soil Texas – –
027 Soil Texas – –
045 Soil Texas – –
034 Soil Texas – –
039 Soil Texas – –

*Ct, cycle threshold; –, negative result, no fluorescence detected after 45 cycles of amplification.
†See Kugeler et al. (10).
‡See Barns et al (9).

*Ct, cycle threshold; –, negative result, no fluorescence detected after 45 cycles of amplification.
†See Kugeler et al. (10).
‡See Barns et al (9).

Main Article

References
  1. Ellis  J, Oyston  PC, Green  M, Titball  RW. Tularemia. Clin Microbiol Rev. 2002;15:631–46. DOIPubMedGoogle Scholar
  2. Dennis  DT, Inglesby  TV, Henderson  DA, Bartlett  JG, Ascher  MS, Eitzen  E, Tularemia as a biological weapon: medical and public health management. JAMA. 2001;285:2763–73. DOIPubMedGoogle Scholar
  3. Sjostedt  A. Family XVII. Francisellaceae, genus I. Francisella. In: Brenner DJ, editor. Bergey’s manual of systematic bacteriology. New York: Springer-Verlag; 2005.
  4. Olsufjev  NG, Meshcheryakova  IS. Infraspecific taxonomy of tularemia agent Francisella tularensis McCoy et Chapin. J Hyg Epidemiol Microbiol Immunol. 1982;26:291–9.PubMedGoogle Scholar
  5. Versage  JL, Severin  DD, Chu  MC, Petersen  JM. Development of a multitarget real-time TaqMan PCR assay for enhanced detection of Francisella tularensis in complex specimens. J Clin Microbiol. 2003;41:5492–9. DOIPubMedGoogle Scholar
  6. Nano  FE, Zhang  N, Cowley  SC, Klose  KE, Cheung  KK, Roberts  MJ, A Francisella tularensis pathogenicity island required for intramacrophage growth. J Bacteriol. 2004;186:6430–6. DOIPubMedGoogle Scholar
  7. Larsson  P, Oyston  PC, Chain  P, Chu  MC, Duffield  M, Fuxelius  HH, The complete genome sequence of Francisella tularensis, the causative agent of tularemia. Nat Genet. 2005;37:153–9. DOIPubMedGoogle Scholar
  8. Petersen  JM, Schriefer  ME, Carter  LG, Zhou  Y, Sealy  T, Bawiec  D, Laboratory analysis of tularemia in wild-trapped, commercially traded prairie dogs, Texas, 2002. Emerg Infect Dis. 2004;10:419–25.PubMedGoogle Scholar
  9. Barns  SM, Grow  CC, Okinaka  RT, Keim  P, Kuske  CR. Detection of diverse new Francisella-like bacteria in environmental samples. Appl Environ Microbiol. 2005;71:5494–500. DOIPubMedGoogle Scholar
  10. Kugeler  KJ, Gurfield  N, Creek  JG, Mahoney  KS, Versage  JL, Petersen  JM. Discrimination between Francisella tularensis and Francisella-like endosymbionts when screening ticks by PCR. Appl Environ Microbiol. 2005;71:7594–7. DOIPubMedGoogle Scholar

Main Article

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