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Volume 12, Number 11—November 2006
Letter

Real-time PCR for Francisella tularensis Types A and B

Kiersten J. Kugeler*, Ryan Pappert*, Yan Zhou*, and Jeannine M. Petersen*Comments to Author 
Author affiliations: *Centers for Disease Control and Prevention, Fort Collins, Colorado, USA

Main Article

Table A1

Specificity evaluation with DNA from Francisella spp

Organism Sample ID Source Geographic origin Type A assay
(Ct value)* Type B assay
(Ct value)*
F. tularensis subsp.
tularensis (type A)
SchuS4 Human Ohio 15.9
WY963418 Human Wyoming 15.9
MA002987 Human Massachusetts 18.4
CO012364 Cat Colorado 18.3
CO013713 Rabbit Colorado 16.5
ND000952 Human North Dakota 16.9
KS000948 Cat Kansas 16.7
OK002731 Human Oklahoma 18.4
NC993990 Rabbit North Carolina 16.8
AR000028 Human Arkansas 18.8
UT983134 Human Utah 16.9
NM990295 Rabbit New Mexico 17.2
NC973057 Rabbit North Carolina 16.5
NC015379 Cat North Carolina 18.6
AR982146 Rabbit Arkansas 18.0
OK004337 Human Oklahoma 18.4
AR011117 Human Arkansas 22.9
MO011907 Human Missouri 20.3

CA020099
Human
California
18.2

F. tularensis subsp.
holarctica (type B)
LVS Rat Russia 16.0
KY993387 Human Kentucky 19.7
OR960246 Monkey Oregon 16.7
CN985979 Human Canada 17.1
AZ001325 Rat Arizona 16.2
IL003633 Human Illinois 18.4
MO011673 Human Missouri 16.8
KY001708 Human Kentucky 15.8
OH013029 Prairie dog Ohio 16.7
UT002098 Human Missouri 18.2
SP986120 Rabbit Spain 17.5
IN983055 Rat Indiana 15.6
CO961243 Vole Colorado 15.5
CA990837 Human California 16.5
IN002758 Human Indiana 15.8
AZ001324 Squirrel Arizona 15.9
CA993992 Monkey California 16.9
SP982108 Human Spain 17.8
NM002642 Human New Mexico 17.9
JAP5-3-11 Human Japan 18.6
KO971026 Human Korea 19.0
F. tularensis subsp.
novicida
GA993548 Human Louisiana
GA993549 Human California
GA993550 Water Utah
UT014992 Human Utah
AS020814 Human Australia
FX1 Human Texas

FX2
Human
Texas


F. philomiragia
GA012793 Human California
GA012794 Human Colorado
GA012795 Human New York
GA012796 Human California
GA012797 Human Pennsylvania
GA012799 Human Connecticut
GA012800 Human Connecticut
GA012801 Human New York
GA012802 Human California
GA012803 Human New Mexico
GA012804 Human Virginia
GA012806 Human Massachusetts
GA012810 Water Utah
GA012811 Water Utah

ATCC 25015
Muskrat
Utah


Francisella-like
tick endosymbionts†
2040372 Tick California
2040460 Tick California

MV2
Tick
Massachusetts


Francisella-like
bacteria‡
005 Soil Texas
013 Soil Texas
015 Soil Texas
027 Soil Texas
045 Soil Texas
034 Soil Texas
039 Soil Texas

*Ct, cycle threshold; –, negative result, no fluorescence detected after 45 cycles of amplification.
†See Kugeler et al. (10).
‡See Barns et al (9).

*Ct, cycle threshold; –, negative result, no fluorescence detected after 45 cycles of amplification.
†See Kugeler et al. (10).
‡See Barns et al (9).

Main Article

References
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