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Volume 12, Number 2—February 2006
Dispatch

Sequencing and Staphylococci Identification

Alexander Mellmann*Comments to Author , Karsten Becker*, Christof von Eiff*, Ursula Keckevoet*, Peter Schumann†, and Dag Harmsen*
Author affiliations: *University Hospital Münster, Münster, Germany; †Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany

Main Article

Table 2

Identification of 55 clinical staphylococcal isolates by using RNA polymerase B (rpoB) gene sequencing

Strain rpoB gene (% similarity*) Definitive identification†
M01 Staphylococcus arlettae (100.0) S. arlettae
M02 S. aureus subsp. aureus (100.0) S. aureus subsp. aureus
M03 S. aureus subsp. aureus (100.0) S. aureus subsp. aureus
M04 S. aureus subsp. aureus (99.8) S. aureus subsp. aureus
M05‡ S. aureus subsp. aureus (99.8) S. aureus subsp. aureus
M06 S. aureus subsp. aureus (100.0) S. aureus subsp. aureus
M07‡ S. aureus subsp. aureus (100.0) S. aureus subsp. aureus
M08 S. haemolyticus (94.0) S. haemolyticus
M09 S. epidermidis (100.0) S. epidermidis
M10 S. capitis subsp. capitis (100.0) S. capitis subsp. capitis
M11 S. epidermidis (100.0) S. epidermidis
M12‡ S. epidermidis (100.0) S. epidermidis
M13‡ S. capitis subsp. capitis (99.8) S. capitis subsp. capitis
M14 S. caprae (99.8) S. caprae
M15 S. caprae (99.8) S. caprae
M16 S. chromogenes (100.0) S. chromogenes
M17 S. cohnii subsp. cohnii (99.8) S. cohnii subsp. cohnii
M18 S. cohnii subsp. cohnii (99.8) S. cohnii subsp. cohnii
M20 S. saprophyticus subsp. saprophyticus (100.0) S. saprophyticus subsp. bovis
M21 S. epidermidis (99.0) S. epidermidis
M22 S. epidermidis (100.0) S. epidermidis
M23‡ S. epidermidis (100.0) S. epidermidis
M24 S. epidermidis (100.0) S. epidermidis
M25‡ S. epidermidis (100.0) S. epidermidis
M26 S. equorum subsp. equorum (100.0); S. equorum subsp. linens (100.0) S. equorum; subspecies not known
M27 S. felis (99.8) S. felis
M28 S. haemolyticus (100.0) S. haemolyticus
M29 S. haemolyticus (99.8) S. haemolyticus
M30 S. epidermidis (100.0) S. epidermidis
M31 S. epidermidis (100.0) S. epidermidis
M32 S. hyicus (100.0) S. hyicus
M33 S. intermedius (100.0) S. intermedius
M34 S. intermedius (100.0) S. intermedius
M35 S. intermedius (100.0) S. intermedius
M36 S. xylosus (100.0) S. xylosus
M37 S. lugdunensis (100.0) S. lugdunensis
M38 S. lugdunensis (100.0) S. lugdunensis
M39 S. saprophyticus subsp. saprophyticus (100.0) S. saprophyticus subsp. bovis
M40 S. aureus subsp. aureus (100.0) S. aureus subsp. aureus
M41 S. schleiferi subsp. schleiferi (100.0) S. schleiferi subsp. schleiferi
M42 S. schleiferi subsp. schleiferi (100.0) S. schleiferi subsp. schleiferi
M43 S. sciuri subsp. sciuri (99.8) S. sciuri subsp. sciuri
M44 S. sciuri subsp. sciuri (99.8) S. sciuri subsp. sciuri
M45 S. sciuri subsp. sciuri (100.0) S. sciuri subsp. sciuri
M46 S. simulans (100.0) S. simulans
M47 S. hominis subsp. novobiosepticus (99.6) S. hominis subsp. novobiosepticus
M48 S. felis (99.8) S. felis
M49 S. felis (99.8) S. felis
M50 S. warneri (95.9) S. warneri
M51 S. warneri (95.3) S. warneri
M52 S. warneri (96.0) S. warneri
M53 S. equorum subsp. equorum (99.8); S. equorum subsp. linens (99.8) S. equorum; subspecies not known
M54 S. xylosus (99.0) S. xylosus
M55 S. xylosus (97.1) S. xylosus
M56 S. xylosus (98.6) S. xylosus

*Similarity in comparison with the reference database.
†By phenotypic and genotypic methods as previously published (4).
‡Isolate exhibiting the small colony variant phenotype.

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