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Volume 12, Number 3—March 2006

Rickettsia prowazekii and Real-time Polymerase Chain Reaction

Sanela Svraka*, Jean-Marc Rolain*, Yassina Bechah*, John Gatabazi†, and Didier Raoult*Comments to Author 
Author affiliations: *Université de la Méditerranée, Marseilles, France; †National Reference Laboratory, Kigali, Rwanda

Main Article


Strains used in real-time PCR*

Strains Source Standard PCR LC PCR assay
Rickettsia prowazekii Breinl ATCC + +
R. prowazekii Evir UR + +
R. prowazekii BatnaRp22 UR + +
R. prowazekii Russian sample UR + +
R. typhi Wilmington ATCC +
R. massiliae MtuI ATCC +
R. montanensis ATCC +
"R. aeschlimanii" UR +
R. massiliae strain Bar 29 UR +
R. helvetica C6P9 ATCC +
R. felis UR +
"R. sibirica mongolitimonae" UR +
R. rickettsii ATCC +
R. conorii moroccan ATCC +
R. sibirica sibirica 246 ATCC +
R. conorii subsp. israelensis CDC1 G.A. Dasch +
R. africae ESF–5 UR +
R. japonica YM ATCC +
Thai tick typhus rickettsia G.A. Dasch +
R. slovaca UR +
R. conorii subsp. caspia A-167 UR +
R. australis Phillips G.A. Dasch +
R. honei RB GRIC +
Rickettsia sp. AT1 UR +
R. bellii 369L42-1 D.H. Walker +
31 lice from Rwanda (2004) J. Bosco + (10/31)† +(17/31)†
30 lice from Rwanda (2005) J. Gatabazi ND +(5/30)
R. prowazekii laboratory-infected lice UR + (10/10) +(10/10)
R. typhi laboratory-infected lice UR + (10/10)
R. prowazekii–infected BALB/C mice UR + (12/12)‡ + (12/12)‡
B. recurrentis laboratory-infected lice UR – (0/10) – (0/10)
Borrelia recurrentis ATCC
Escherichia coli CIP
Proteus mirabilis CIP
Staphylococcus aureus CIP
Streptococcus salivarius CIP
Orientia tsutsugamushi CIP +
Streptococcus pyogenes CIP
Mycobacterium xenopi CIP
Chlamydia trachomatis Human isolate
Propionibacterium acnes UR
HGE agent ATCC
Bartonella quintana Oklahoma ATCC
Tropheryma whipplei Twist UR
M. tuberculosis CIP

*PCR, polymerase chain reaction; LC, LightCycler; ATCC, American Type Culture Collection, Rockville, MD, USA; GRIC, Gamaleya Research Institute Collection; G.A. Dasch, Naval Medical Research Institute, Bethesda, MD, USA; UR, Unité des Rickettsies, CNRS UPRES A, Marseille, France; D.H. Walker, University of Texas, Galveston; CIP, Collection Institute Pasteur, Paris, France; HGE, human granulocytic ehrlichiosis.
†Number of positive lice/total number of tested lice.
‡PCR for each mouse was positive in blood at days 3 and 6 postinfection (for cycle thresholds see results in text).

Main Article

Page created: January 27, 2012
Page updated: January 27, 2012
Page reviewed: January 27, 2012
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