In June 2003, an Epomophorus wahlbergi carcass was recovered in Durban, KwaZulu-Natal, after the bat was caught by a domestic cat. In August 2004, a resident of Umbilo, Durban, found a dead E. wahlbergi fruit bat on her lawn one morning after hearing squeaking noises around the house during the night. The fluorescent-antibody test (FAT), performed on brain material, was positive for lyssavirus antigens, and virus was isolated in both cases when suckling mice died 9–14 days after intracerebral injection with brain suspensions. Antigenic typing was carried out with a panel of anti-lyssavirus nucleocapsid monoclonal antibodies (prepared by the Centre of Expertise for Rabies, Canadian Food Inspection Agency, Nepean, Ontario, Canada). These analyses identified both new isolates as LBV (genotype 2) (Table 2). Additional characterization was accomplished by polymerase chain reaction (PCR) and sequencing of a 457-bp region of the nucleoprotein-encoding gene with a novel set of PCR and sequencing primers specific for LBV (LagNF (5´-GGGCAGATATGACGCGAGA-3´) and LagNR (5´-TTGACCGGGTTCAAACATC-3´). Briefly, total RNA was extracted from infected tissue by using TRIzol (Invitrogen, Croningen, the Netherlands) according to the manufacturer's instructions. Complementary DNA was produced by a reverse transcription reaction (RT) and used in subsequent PCR. PCR products were purified by using the Wizard SV PCR and Gel purification kit (Promega, Madison, WI, USA). The purified products were then sequenced by using the Big Dye Termination Cycle Sequencing Ready Reaction Kit 3.1 (Applied Biosystems, Foster City, CA, USA), according to the manufacturer's protocol, with subsequent analysis on an Applied Biosystems 377 DNA automated sequencer.

In June 2005, a caretaker/gardener at a communal outdoor sports complex in the Bluff, Durban, found a bat on the lawns of the complex. At the time, birds were picking at it, and on closer inspection, it was found to be an immobile adult animal with a pup attached to it. The caretaker collected both bats and placed them in a nearby tree. Later, the bats, still attached to each other, were again found on the ground, where eyewitnesses also saw a cat toying with it. The animals were then presented to a local bat rehabilitator. The adult animal died and was submitted for diagnostic testing, but results of FAT carried out on brain smears were repeatedly negative. The pup had at least 1 evident bite wound, presumably from the cat, but otherwise appeared healthy and was cared for by the rehabilitator. Although the pup was reported to be feeding and doing well, it suddenly died ≈4 days after being found, on June 21, 2005. By this time, RT-PCR and nucleotide sequencing assays, carried out as described above, showed LBV in brain material from the adult. Antigenic typing was not performed because the level of lyssavirus antigen in the brain matter was undetectable. In the meantime, the carcass of the pup was recovered, and brain material was subjected to FAT and diagnostic RT-PCR. Although the RT-PCR results were inconclusive, the FAT results were negative.

Figure

Figure. A neighbor-joining tree comparing 457 nucleotides of the nucleoprotein-encoding genes of the new Lagos bat isolations made in South Africa (bat 2003 [DQ201178], 2004 [DQ201179], and 2005 [DQ201180]) with representative sequences...

DNA sequencing information from each case was compared with nucleoprotein sequence information for LBV and other lyssavirus species (genotypes) available in the public domain (GenBank). ClustalW was used to produce sequence alignments and generate a phylogenetic tree (Figure). A graphic representation of the trees was constructed with the TreeView program. In this phylogeny, the 3 new LBV isolates segregate together with previously identified LBV isolates from Ethiopia (AY333110) (7) and Nigeria (U22842) (5). The recent isolates from South Africa share a close sequence homology with the isolate from Ethiopia. This finding warrants further investigation.

Although LBV is rare and has not been reported in South Africa in 13 years, a small-scale passive surveillance effort in KwaZulu-Natal, South Africa, enabled us to identify 3 new isolations of LBV in a relatively short time. This finding reemphasizes our lack of understanding of the true prevalence of lyssaviruses in Africa because of poor surveillance for non-rabies viruses (and, in fact, RABV) throughout the continent. Human infections with LBV have not been documented to date; however, this virus has been reported in domestic animals (2 cats [4] and a dog [5]). We describe close contact between humans and other animals and LBV-infected bats. Cross-neutralization data obtained in rodent models show that rabies preexposure and postexposure prophylaxis is unlikely to be effective against LBV (14). We have shown that LBV infection may be present in bat populations; consequently, we recommend appropriate precautions and use of proper personal protection equipment, such as gloves, when interacting with these animals. Even though the value of rabies vaccination is doubtful, it should be considered in light of the potential for cross-reactivity (15) and the lack of alternatives. Surveillance should be maintained as part of a strategy to better understand the epidemiology of LBV. Cumulatively, all available evidence indicates that LBV is likely persistently maintained in Megachiroptera populations in South Africa and other African countries where LBV has been reported in the past.