Volume 12, Number 4—April 2006
Research
Atypical Enteropathogenic Escherichia coli Infection and Prolonged Diarrhea in Children
Table 1
Interpretation† | Gene or virulence-associated determinant* |
||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
pCVD432 | aggA | bfpA | eae | ipaC | stIA | ltA | ehxA | stx1 | stx2 | Control strain (reference) | |
EAEC‡ | + | + | – | – | – | – | – | – | – | – | O42 (13) |
Typical EPEC | – | – | + | + | – | – | – | – | – | – | E2348/69 (14) |
Atypical EPEC | – | – | – | + | – | – | – | – | – | – | E128012 (14) |
EIEC | – | – | – | – | + | – | – | – | – | – | 223/83 (15) |
ETEC‡ | – | – | – | – | – | + | + | – | – | – | H10407 (16) |
EHEC§ | – | – | – | + | – | – | – | + | + | + | EDL933 (17) |
STEC not EHEC¶ | – | – | – | – | – | – | – | – | + | + |
*Factors specified by virulence genes: aggA, aggregative fimbria, AAF/I; bfpA, bundle-forming pilus; eae, intimin; ipaC, invasion plasmid antigen; stIA, heat-stable enterotoxin; ltA, heat-labile enterotoxin; ehxA, EHEC hemolysin; stx, Shiga toxin.
†EAEC, enteroaggregative E. coli; EPEC, enteropathogenic E. coli; EIEC, enteroinvasive E. coli; ETEC, enterotoxigenic E. coli; STEC, Shiga toxin–producing E. coli; EHEC, enterohemorrhagic E. coli.
‡Either positive by polymerase chain reaction.
§Either eae or ehxA, and either stx1 or stx2 positive.
¶Either stx1 or stx2 positive.
References
- Nataro JP, Kaper JB. Diarrheagenic Escherichia coli. Clin Microbiol Rev. 1998;11:142–201.PubMedGoogle Scholar
- Robins-Browne RM, Hartland EL. Escherichia coli as a cause of diarrhea. J Gastroenterol Hepatol. 2002;17:467–75. DOIPubMedGoogle Scholar
- Robins-Browne RM. Traditional enteropathogenic Escherichia coli of infantile diarrhea. Rev Infect Dis. 1987;9:28–53. DOIPubMedGoogle Scholar
- Trabulsi LR, Keller R, Gomes TAT. Typical and atypical enteropathogenic Escherichia coli. Emerg Infect Dis. 2002;8:508–3.PubMedGoogle Scholar
- Bieber D, Ramer SW, Wu CY, Murray WJ, Tobe T, Fernandez R, Type IV pili, transient bacterial aggregates, and virulence of enteropathogenic Escherichia coli. Science. 1998;280:2114–8. DOIPubMedGoogle Scholar
- Frankel G, Phillips AD, Rosenshine I, Dougan G, Kaper JB, Knutton S. Enteropathogenic and enterohaemorrhagic Escherichia coli: more subversive elements. Mol Microbiol. 1998;30:911–21. DOIPubMedGoogle Scholar
- Gomez-Duarte OG, Kaper JB. A plasmid-encoded regulatory region activates chromosomal eaeA expression in enteropathogenic Escherichia coli. Infect Immun. 1995;63:1767–76.PubMedGoogle Scholar
- Hellard ME, Sinclair MI, Forbes AB, Fairley CK. A randomized, blinded, controlled trial investigating the gastrointestinal health effects of drinking water quality. Environ Health Perspect. 2001;109:773–8. DOIPubMedGoogle Scholar
- Robins-Browne RM, Bordun A-M, Tauschek M, Bennett-Wood V, Russell J, Oppedisano F, Atypical enteropathogenic Escherichia coli: a leading cause of community-acquired gastroenteritis in Melbourne, Australia. Emerg Infect Dis. 2004;10:1797–805.PubMedGoogle Scholar
- Ruuska T, Vesikari T. Rotavirus disease in Finnish children: use of numerical scores for clinical severity of diarrhoeal episodes. Scand J Infect Dis. 1990;22:259–67. DOIPubMedGoogle Scholar
- Garcia LS. Diagnostic medical parasitology, 4th ed. Washington: ASM Press; 2001.
- Barnes GL, Uren E, Stevens KB, Bishop RF. Etiology of acute gastroenteritis in hospitalized children in Melbourne, Australia, from April 1980 to March 1993. J Clin Microbiol. 1998;36:133–8.PubMedGoogle Scholar
- Vial P, Robins-Browne R, Lior H, Prado V, Kaper JB, Nataro JP, Characterization of enteroadherent-aggregative Escherichia coli, a putative agent of diarrheal disease. J Infect Dis. 1988;158:70–9. DOIPubMedGoogle Scholar
- Levine MM, Nataro JP, Karch H, Baldini MM, Kaper JB, Black RE, The diarrheal response of humans to some classic serotypes of enteropathogenic Escherichia coli is dependent on a plasmid encoding an enteroadhesiveness factor. J Infect Dis. 1985;152:550–9. DOIPubMedGoogle Scholar
- Wood PK, Morris JG Jr, Small PLC, Sethabutr O, Toledo MRF, Trabulsi L, Comparison of DNA probes and the Sereny test for identification of invasive Shigella and Escherichia coli strains. J Clin Microbiol. 1986;24:498–500.PubMedGoogle Scholar
- Satterwhite TK, Evans DG, DuPont HL, Evans DJ Jr. Role of Escherichia coli colonization factor antigen in acute diarrhoea. Lancet. 1978;ii:181–4. DOIGoogle Scholar
- Perna NT, Plunkett G, Burland V, Mau B, Glasner JD, Rose DJ, Genome sequence of enterohaemorrhagic Escherichia coli O157:H7. Nature. 2001;409:529–33. DOIPubMedGoogle Scholar
- Bettelheim KA, Thompson CJ. New method of serotyping Escherichia coli: implementation and verification. J Clin Microbiol. 1987;25:781–6.PubMedGoogle Scholar
- Robins-Browne RM. Seasonal and racial incidence of infantile gastroenteritis in South Africa. Am J Epidemiol. 1984;119:350–5.PubMedGoogle Scholar
- Afset JE, Bergh K, Bevanger L. High prevalence of atypical enteropathogenic Escherichia coli (EPEC) in Norwegian children with diarrhoea. J Med Microbiol. 2003;52:1015–9. DOIPubMedGoogle Scholar
- Vieira MA, Andrade JR, Trabulsi LR, Rosa AC, Dias AM, Ramos SR, Phenotypic and genotypic characteristics of Escherichia coli strains of non-enteropathogenic E. coli (EPEC) serogroups that carry EAE and lack the EPEC adherence factor and Shiga toxin DNA probe sequences. J Infect Dis. 2001;183:762–72. DOIPubMedGoogle Scholar
- Blaser MJ, Smith PD, Ravdin JI, Greenberg HB, Guerrant RL. Infections of the gastrointestinal tract. 2nd ed. New York: Raven Press; 2002.
- Walker-Smith JA. Post-infective diarrhoea. Curr Opin Infect Dis. 2001;14:567–71. DOIPubMedGoogle Scholar
- Nataro JP, Sears CL. Infectious causes of persistent diarrhea. Pediatr Infect Dis J. 2001;20:195–6. DOIPubMedGoogle Scholar
- Viljanen MK, Peltola T, Junnila SY, Olkkonen L, Jarvinen H, Kuistila M, Outbreak of diarrhoea due to Escherichia coli O111:B4 in schoolchildren and adults: association of Vi antigen-like reactivity. Lancet. 1990;336:831–4. DOIPubMedGoogle Scholar
- Yatsuyanagi J, Saito S, Miyajima Y, Amano K, Enomoto K. Characterization of atypical enteropathogenic Escherichia coli strains harboring the astA gene that were associated with a waterborne outbreak of diarrhea in Japan. J Clin Microbiol. 2003;41:2033–9. DOIPubMedGoogle Scholar
- Bokete TN, Whittam TS, Wilson RA, Clausen CR, O'Callahan CM, Moseley SL, Genetic and phenotypic analysis of Escherichia coli with enteropathogenic characteristics isolated from Seattle children. J Infect Dis. 1997;175:1382–9. DOIPubMedGoogle Scholar
- Bouzari S, Jafari MN, Shokouhi F, Parsi M, Jafari A. Virulence-related DNA sequences and adherence patterns in strains of enteropathogenic Escherichia coli. FEMS Microbiol Lett. 2000;185:89–93. DOIPubMedGoogle Scholar
- Galane PM, Le Roux M. Molecular epidemiology of Escherichia coli isolated from young South African children with diarrhoeal diseases. J Health Popul Nutr. 2001;19:31–8.PubMedGoogle Scholar
- Knutton S, Shaw R, Phillips AD, Smith HR, Willshaw GA, Watson P, Phenotypic and genetic analysis of diarrhea-associated Escherichia coli isolated from children in the United Kingdom. J Pediatr Gastroenterol Nutr. 2001;33:32–40. DOIPubMedGoogle Scholar
- Nataro JP, Baldini MM, Kaper JB, Black RE, Bravo N, Levine MM. Detection of an adherence factor of enteropathogenic Escherichia coli with a DNA probe. J Infect Dis. 1985;152:560–5. DOIPubMedGoogle Scholar
- Paciorek J. Virulence properties of Escherichia coli faecal strains isolated in Poland from healthy children and strains belonging to serogroups O18, O26, O44, O86, O126 and O127 isolated from children with diarrhoea. J Med Microbiol. 2002;51:548–56.PubMedGoogle Scholar
- Kukuruzovic R, Robins-Browne RM, Anstey NM, Brewster DR. Enteric pathogens, intestinal permeability and nitric oxide production in acute gastroenteritis. Pediatr Infect Dis J. 2002;21:730–9. DOIPubMedGoogle Scholar
- Afset JE, Bevanger L, Romundstad P, Bergh K. Association of atypical enteropathogenic Escherichia coli (EPEC) with prolonged diarrhoea. J Med Microbiol. 2004;53:1137–44. DOIPubMedGoogle Scholar
- Hill SM, Phillips AD, Walker-Smith JA. Enteropathogenic Escherichia coli and life threatening chronic diarrhoea. Gut. 1991;32:154–8. DOIPubMedGoogle Scholar
- Scotland SM, Smith HR, Rowe B. Escherichia coli O128 strains from infants with diarrhea commonly show localized adhesion and positivity in the fluorescent-actin staining test but do not hybridize with an enteropathogenic E. coli probe. Infect Immun. 1991;59:1569–71.PubMedGoogle Scholar
- Mellmann A, Bielaszewska M, Zimmerhackl LB, Prager R, Harmsen D, Tschäpe H, Enterohemorrhagic Escherichia coli in human infection: in vivo evolution of a bacterial pathogen. Clin Infect Dis. 2005;41:785–92. DOIPubMedGoogle Scholar
- Chen HD, Frankel G. Enteropathogenic Escherichia coli: unravelling pathogenesis. FEMS Microbiol Rev. 2005;29:83–98. DOIPubMedGoogle Scholar
- Heczko U, Carthy CM, O'Brien BA, Finlay BB. Decreased apoptosis in the ileum and ileal Peyer's patches: a feature after infection with rabbit enteropathogenic Escherichia coli O103. Infect Immun. 2001;69:4580–9. DOIPubMedGoogle Scholar
- Melo AR, Lasunskaia EB, de Almeida CM, Schriefer A, Kipnis TL. as da SW. Expression of the virulence factor, BfpA, by enteropathogenic Escherichia coli is essential for apoptosis signalling but not for NF-kappaB activation in host cells. Scand J Immunol. 2005;61:511–9. DOIPubMedGoogle Scholar
1Present address: An Giang General Hospital, Long Xuyen City, An Giang, Vietnam.
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