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Volume 12, Number 5—May 2006


Shiga-toxigenic Escherichia coli O157 in Agricultural Fair Livestock, United States

James E. Keen*Comments to Author , Thomas E. Wittum†, John R. Dunn‡, James L. Bono*, and Lisa M. Durso*
Author affiliations: *United States Department of Agriculture, Clay Center, Nebraska, USA; †Ohio State University, Columbus, Ohio, USA; ‡Louisiana State University, Baton Rouge, Louisiana, USA

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Table 3

PFGE patterns (n = 47) of 79 STEC O157:H7 from animal fecal, fly, and environmental samples at 7 selected state and county fairs, 2002 (livestock and fly isolates) and 2003 (environmental isolates)*

Fair code Total isolates PFGE patterns Unique XbaI patterns (P1–P47) (sample type† - no. isolates) Simpson D (95% CI)‡
SF2 35 25 P1 (F-5, B-2), P2 (B-2), P3 (B-2), P4 (B-2), P5 (B-2), P6–P25§ 0.96 (0.92–0.99)
CF19 4 2 P26 (B-1, P-1), P27 (E-2) 0.67 (0.49–0.85)
CF20 9 5 P28 (P-1, B-1, F-1), P29 (F-3), P30 (D-1),¶ P31 (B-1), P32 (D-1) 0.83 (0.73–0.94)
CF22 14 6 P33 (B-5, P-2), P34 (S-3), P30 (D-1),¶ P35 (B-1), P36 (B-1), P37 (B-1) 0.74 (0.59–0.89)
CFB 4 4 P38 (Ef-1), P39 (B-1), P40 (B-1), P41 (S-1) 1.00 (NA)
CFY 4 4 P42 (B-1), P43 (B-1), P44 (B-1), P45 (S-1), 1.00 (NA)
CF16 9 2 P46 (B-8),# P47 (B-1) 0.22 (0.00–0.57)

*PFGE, pulsed-field gel electrophoresis; STEC, Shiga-toxigenic Escherichia coli.
†F, fly (pooled fly sample collected when livestock were present on fair grounds); B, beef cattle; P, pig; E, environment; D, dairy cattle; S, sheep; Ef, environment fly (pooled fly sample collected when fair grounds were closed to the public and no animals were present).
‡Probability that any 2 randomly selected isolates from a given fair have unique PFGE patterns. CI, confidence interval; NA, not available.
§Twenty isolates from SF2 each with a unique PFGE pattern from the following samples: 16 beef cattle, 2 sheep, 1 pig, and 1 dairy cow.
¶Isolates from dairy samples at CF20 and CF22 were indistinguishable; fairs were geographically close to each other.
#A Stool STEC O157 isolate from a human clinical case-patient (hemorrhagic colitis) who had visited this fair also had this PFGE pattern.

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