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Volume 12, Number 5—May 2006

Shiga-toxigenic Escherichia coli O157 in Agricultural Fair Livestock, United States

James E. Keen*Comments to Author , Thomas E. Wittum†, John R. Dunn‡, James L. Bono*, and Lisa M. Durso*
Author affiliations: *United States Department of Agriculture, Clay Center, Nebraska, USA; †Ohio State University, Columbus, Ohio, USA; ‡Louisiana State University, Baton Rouge, Louisiana, USA

Main Article

Table 3

PFGE patterns (n = 47) of 79 STEC O157:H7 from animal fecal, fly, and environmental samples at 7 selected state and county fairs, 2002 (livestock and fly isolates) and 2003 (environmental isolates)*

Fair code Total isolates PFGE patterns Unique XbaI patterns (P1–P47) (sample type† - no. isolates) Simpson D (95% CI)‡
SF2 35 25 P1 (F-5, B-2), P2 (B-2), P3 (B-2), P4 (B-2), P5 (B-2), P6–P25§ 0.96 (0.92–0.99)
CF19 4 2 P26 (B-1, P-1), P27 (E-2) 0.67 (0.49–0.85)
CF20 9 5 P28 (P-1, B-1, F-1), P29 (F-3), P30 (D-1),¶ P31 (B-1), P32 (D-1) 0.83 (0.73–0.94)
CF22 14 6 P33 (B-5, P-2), P34 (S-3), P30 (D-1),¶ P35 (B-1), P36 (B-1), P37 (B-1) 0.74 (0.59–0.89)
CFB 4 4 P38 (Ef-1), P39 (B-1), P40 (B-1), P41 (S-1) 1.00 (NA)
CFY 4 4 P42 (B-1), P43 (B-1), P44 (B-1), P45 (S-1), 1.00 (NA)
CF16 9 2 P46 (B-8),# P47 (B-1) 0.22 (0.00–0.57)

*PFGE, pulsed-field gel electrophoresis; STEC, Shiga-toxigenic Escherichia coli.
†F, fly (pooled fly sample collected when livestock were present on fair grounds); B, beef cattle; P, pig; E, environment; D, dairy cattle; S, sheep; Ef, environment fly (pooled fly sample collected when fair grounds were closed to the public and no animals were present).
‡Probability that any 2 randomly selected isolates from a given fair have unique PFGE patterns. CI, confidence interval; NA, not available.
§Twenty isolates from SF2 each with a unique PFGE pattern from the following samples: 16 beef cattle, 2 sheep, 1 pig, and 1 dairy cow.
¶Isolates from dairy samples at CF20 and CF22 were indistinguishable; fairs were geographically close to each other.
#A Stool STEC O157 isolate from a human clinical case-patient (hemorrhagic colitis) who had visited this fair also had this PFGE pattern.

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Page created: January 12, 2012
Page updated: January 12, 2012
Page reviewed: January 12, 2012
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.