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Volume 13, Number 2—February 2007


Surveillance for Shiga Toxin–producing Escherichia coli, Michigan, 2001–2005

Shannon D. Manning*, Robbie T. Madera†, William Schneider†, Stephen E. Dietrich†, Walid Khalife‡, William Brown§, Thomas S. Whittam*, Patricia Somsel†, and James T. Rudrik†Comments to Author 
Author affiliations: *Michigan State University, East Lansing, Michigan, USA; †Michigan Department of Community Health, Lansing, Michigan, USA; ‡Sparrow Health System, Lansing, Michigan, USA; §Detroit Medical Center, Detroit, Michigan, USA;

Main Article

Table 1

Evaluation of shiga toxin–producing Escherichia coli detection methods, Michigan, 2001–2005*

Detection methodStool samplesRoutine STEC surveillance†, 
no. (%)
Enhanced STEC surveillance, 
no. (%)
Culture and PCR‡ for stx genes of suspect NSF E. coliTotal327337249317286
O15785 (26)94 (28)63 (25)58 (18)56 (20)
Non-O1572 (0.6)0 (0)0 (0)2 (0.6)1 (0.3)
EIA of bloody or suspect samplesTotalN/AN/A20914173
O15741111 (5)7 (5)6 (8)
Non-O157494 (2)7 (5)7 (10)
EIA of all samplesTotalN/AN/AN/A1,4051,021
O157N/AN/AN/A5 (0.4)1 (0.1)
Non-O157N/AN/AN/A1 (0.07)0 (0)

*STEC, Shiga toxin–producing Escherichia coli; NSF, non-sorbitol–fermenting; EIA, enzyme immunoassay.
†In 2001 and 2002, EIA of bloody stools was performed by 2 large clinical laboratories in Michigan and was not part of the enhanced STEC surveillance system at the Michigan Department of Community Health. Therefore, the total number of EIAs performed is not known; the total number of EIA s submitted as positive for Stx is provided by serotype.
‡PCR for stx1,2 genes was not initiated at the Michigan Department of Community Health until 2002. Before 2002, a DNA probe (Digene Signal Kit, Digene Diagnostic, Inc., Silver Spring, MD, USA) was used to detect STEC.

Main Article