We studied 4,482 animals (1,966 cattle, 1,237 buffaloes, 813 sheep, and 366 goats) from production and breeding farms in various governorates in Egypt during 2007; the animals had no history of having been tested for brucellosis. Milk and tissue samples obtained from all animals were examined for Brucella spp. We used serologic tests recommended by the National Brucella Committee, which represents the general organization of veterinary services, veterinary laboratories, and universities in Egypt (5). The buffered acidified plate antigen (BAPA) test, the Rose Bengal plate test, the standard tube agglutination test, and the Rivanol test were used as described (6–8).

Direct culture of milk under aseptic conditions was conducted as follows: ≈20 mL of milk was centrifuged at 1,620 × g for 10 min, and the sediment cream mixture was placed on Brucella spp. agar plates containing an antimicrobial drug supplement. Tissue specimens obtained from internal organs, supramammary lymph nodes, and udders were cultured in the same media and incubated at 37°C in an atmosphere of 10% CO₂. Cultured plates were examined for Brucella spp. growth on day 4 and daily for 4 weeks. Suspected colonies were further identified and subcultured on Brucella spp. agar slants. We identified Brucella spp. isolates according to morphologic characteristics, microscopic appearance, and reactions with positive sera. Brucella spp. isolates were typed according to their CO₂ requirement, H₂S production, growth in the presence of dyes, reaction with monospecific sera (immunoglobulin [Ig] A and IgM), and bacteriophage typing (Tiblisi phage; Central Veterinary Laboratory, Wybridge, UK) as described (7).

Results obtained for different animal groups are shown in Table 1. Prevalence of brucellosis in cattle was 5.44% by the BAPA test; highest prevalence was in Benisuef (7.77%) and Monofia (7.14%). Prevalence of brucellosis in buffaloes was 4.11% by the BAPA test; highest prevalence was in Benisuef (6.93%) and Qalioubia (5.34%). Prevalence of brucellosis in sheep was 5.41% by the BAPA test; highest prevalence was in Benisuef (6.91%) and Giza (5.81%). Prevalence of brucellosis in goats was 3.55% by the BAPA test; highest prevalence was in Monofia (6.35%) and Benisuef (5.75%).

Prevalence of a serologic reaction was 4.98% for cattle, 3.52% for buffaloes, 4.8% for sheep, and 2.19% for goats by the Rose Bengal plate test. Prevalence of a serologic reaction was 4.73% for cattle, 3.44% for buffaloes, 4.8% for sheep, and 2.19% for goats by the standard tube agglutination test. Prevalence of a serologic reaction was 4.48% for cattle, 3.37% for buffaloes, 4.8% for sheep, and 2.19% for goats by the Rivanol test. The highest prevalence for cattle, buffaloes, sheep, and goats by any of the 4 tests was in Benisuef, except for the BAPA test in goats, which showed highest prevalence rates in Monofia.

Isolation of the causative agent is still the standard diagnostic method for brucellosis (9). Thus, for definitive and confirmative diagnosis of serologically reactive animals, bacteriologic isolation and identification of Brucella spp. were performed. Results of bacteriologic isolation from milk and tissues all animals are shown in Table 2. A total of 47 isolates of Brucella spp. were identified; all isolates were B. melitensis biovar 3. Isolation of Brucella spp. confirmed active brucellosis in the animals tested. A Brucella spp. was also isolated from milk samples from serologically nonreactive buffaloes in Benisuef.

We observed an increase in animals serologically reactive for Brucella spp. in Egypt in 2007 (Table 1). Prevalence rates in cattle, buffaloes, sheep, and goats were generally higher in Benisuef than in other governorates. Variations in infection in different governorates may be attributed to environmental factors and stress, which may modulate susceptibility to infection.

Increased prevalence of brucellosis in cattle and buffaloes in Egypt can be attributed to raising sheep and goats with cattle or buffaloes in villages. Most sheep or goat flocks in Egypt are mobile. Movement of infected sheep or goats can contaminate pastures and spread brucellosis to other animals (e.g., cattle or buffaloes) in other herds or areas. This movement is a major risk factor for failure of brucellosis eradication programs. Elimination or control of infection in sheep and goat flocks can reduce spread of the disease in cattle and buffaloes.

All Brucella isolates were B. melitensis biovar 3. This finding is consistent with reports of B. melitensis, particularly biovar 3, being the main cause of brucellosis in animals and humans in many countries (5). Isolation and identification of 1 Brucella spp. from milk samples of serologically nonreactive buffaloes in Benisuef emphasize the need to routinely check milk samples. Some microorganisms, which can escape identification by not causing appreciable serologic responses, can localize in the udder and be isolated from milk samples.

We recently reported prevalence of human brucellosis in Egypt as high as 8% in high-risk populations (10). Our findings emphasize the need for continuous national surveillance programs for control and prevention of brucellosis in Egypt and other affected countries. Measures should be established to control spread of brucellosis, especially in mobile flocks. These measures should include identification of infected animals by periodic examination of flocks or newly purchased animals, application of testing and slaughter policies, adoption of vaccination programs, and strict quarantine measures. Sheep farmers should also be notified about transmission of brucellosis from sheep to cattle and buffaloes. Educational programs about brucellosis are important for livestock owners and consumers.