Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 14, Number 12—December 2008
Letter

Candidate New Species of Kobuvirus in Porcine Hosts

Gábor ReuterComments to Author , Ákos Boldizsár, István Kiss, and Péter Pankovics
Author affiliations: ÁNTSZ Regional Institute of State Public Health Service, Pécs, Hungary (G. Reuter, Á. Boldizsár, P. Pankovics); Veterinary Diagnostic Directorate, Debrecen, Hungary (I. Kiss)

Main Article

Figure

Phylogenetic tree of porcine kobuvirus (Kobuvirus/swine/S-1-HUN/2007/Hungary, GenBank accession no. EU787450), based upon the 1,065-nt fragment of the kobuvirus 3C/3D regions. The phylogenetic tree was constructed by using the neighbor-joining clustering method; distance was calculated by using the maximum composite likelihood correction for evolutionary rate with help of the MEGA version 4.0 software (10). Bootstrap values (based on 1,000 replicates) for each node if >50% are given. Reference strains were obtained from GenBank. The human rhinovirus 2 strain (X02316) was included in the tree as an outgroup. Scale bar indicates nucleotide substitutions per site.

Figure. Phylogenetic tree of porcine kobuvirus (Kobuvirus/swine/S-1-HUN/2007/Hungary, GenBank accession no. EU787450), based upon the 1,065-nt fragment of the kobuvirus 3C/3D regions. The phylogenetic tree was constructed by using the neighbor-joining clustering method; distance was calculated by using the maximum composite likelihood correction for evolutionary rate with help of the MEGA version 4.0 software (10). Bootstrap values (based on 1,000 replicates) for each node if >50% are given. Reference strains were obtained from GenBank. The human rhinovirus 2 strain (X02316) was included in the tree as an outgroup. Scale bar indicates nucleotide substitutions per site.

Main Article

References
  1. Online  ICTV. Discussion forum of the International Committee on Taxonomy of Viruses, ICTV 2008 Official Taxonomy [cited 2008 Oct 22]. Available from http://talk.ictvonline.org
  2. Yamashita  T, Kobayashi  S, Sakae  K, Nakata  S, Chiba  S, Ishihara  Y, Isolation of cytopathic small round viruses with BS-C-1 cells from patients with gastroenteritis. J Infect Dis. 1991;164:9547.PubMed
  3. Yamashita  T, Ito  M, Kabashima  Y, Tsuzuki  H, Fujiura  A, Sakae  K. Isolation and characterization of a new species of kobuvirus associated with cattle. J Gen Virol. 2003;84:306977. DOIPubMed
  4. Pham  NT, Khamrin  P, Nguyen  TA, Kanti  DS, Phan  TG, Okitsu  S, Isolation and molecular characterization of Aichi viruses from fecal specimens collected in Japan, Bangladesh, Thailand, and Vietnam. J Clin Microbiol. 2007;45:22878. DOIPubMed
  5. Oh  DY, Silva  PA, Hauroeder  B, Deidrich  S, Cardoso  DD, Schreier  E. Molecular characterization of the first Aichi viruses isolated in Europe and in South America. Arch Virol. 2006;151:1199206. DOIPubMed
  6. Ambert-Balay  K, Lorrot  M, Bon  F, Giraudon  H, Kaplon  J, Wolfer  M, Prevalence and genetic diversity of Aichi virus strains in stool samples from community and hospitalized patients. J Clin Microbiol. 2008;46:12528. DOIPubMed
  7. Khamrin  P, Maneekarn  N, Peerakome  S, Okitsu  S, Mizuguchi  M, Ushijama  H. Bovine kobuviruses from cattle with diarrhea. Emerg Infect Dis. 2008;14:9856. DOIPubMed
  8. Jiang  X, Huang  PW, Zhong  WM, Farkas  T, Cubitt  DW, Matson  DO. Design and evaluation of a primer pair that detects both Norwalk- and Sapporo-like caliciviruses by RT-PCR. J Virol Methods. 1999;83:14554. DOIPubMed
  9. Reuter  G, Krisztalovics  K, Vennema  H, Koopmans  M. Szűcs Gy. Evidence of the etiological predominance of norovirus in gastroenteritis outbreaks—emerging new variant and recombinant noroviruses in Hungary. J Med Virol. 2005;76:598607. DOIPubMed
  10. Tamura  K, Dudley  J, Nei  M, Kumar  S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol. 2007;24:15969. DOIPubMed

Main Article

Page created: July 22, 2010
Page updated: July 22, 2010
Page reviewed: July 22, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external