Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 15, Number 12—December 2009
Dispatch

New Adenovirus in Bats, Germany

Michael Sonntag, Kristin Mühldorfer, Stephanie Speck, Gudrun Wibbelt, and Andreas KurthComments to Author 
Author affiliations: Robert Koch Institute, Berlin, Germany (M. Sonntag, A. Kurth); Leibniz Institute for Zoo and Wildlife Research, Berlin (K. Mühldorfer, S. Speck. G. Wibbelt)

Main Article

Figure 1

A) Electron micrograph of adenovirus particles isolated from Pipistrellus pipistrellus bat 199/07, Germany. Negatively stained with 1% uranyl acetate. Virus particles were 70–90 nm in diameter with an icosahedral shape. Scale bar = 100 nm. B) Schematic representation of the genomic fragments obtained from bat adenovirus 2 (GenBank accession no. FJ983127) in correspondence to canine adenovirus 2 strain Toronto A26/61 (GenBank accession no. U77082). Genomic fragments were generated by generic aden

Figure 1. A) Electron micrograph of adenovirus particles isolated from Pipistrellus pipistrellus bat 199/07, Germany. Negatively stained with 1% uranyl acetate. Virus particles were 70–90 nm in diameter with an icosahedral shape. Scale bar = 100 nm. B) Schematic representation of the genomic fragments obtained from bat adenovirus 2 (GenBank accession no. FJ983127) in correspondence to canine adenovirus 2 strain Toronto A26/61 (GenBank accession no. U77082). Genomic fragments were generated by generic adenovirus-specific PCR (10) and a virus discovery based on cDNA–amplified fragment length polymorphism PCR method (11). Partial sequence of the DNA polymerase gene was generated from LongRange PCR product. Purified PCR products were directly sequenced by using the BigDye Terminator Cycle Sequencing ready Reaction kit (Applied Biosystems, Foster City, CA, USA) and analyzed on an ABI 3770 automatic sequencer (Applied Biosytems). C, clone; P, ≈550-bp nested PCR product.

Main Article

References
  1. Wibbelt  G, Speck  S, Field  H. Methods for assessing diseases in bats. In: Kunz TH, Parsons S, editors. Ecological and behavioral methods for the study of bats. Baltimore (MD): Johns Hopkins University Press; 2009. p. 775–794.
  2. Johnson  N, Wakeley  PR, Brookes  SM, Fooks  AR. European bat lyssavirus type 2 RNA in Myotis daubentonii. Emerg Infect Dis. 2006;12:11424.PubMed
  3. Halpin  K, Young  PL, Field  HE, Mackenzie  JS. Isolation of Hendra virus from pteropid bats: a natural reservoir of Hendra virus. J Gen Virol. 2000;81:192732.PubMed
  4. Chua  KB, Koh  CL, Hooi  PS, Wee  KF, Khong  JH, Chua  BH, Isolation of Nipah virus from Malaysian Island flying-foxes. Microbes Infect. 2002;4:14551. DOIPubMed
  5. Lau  SK, Woo  PC, Li  KS, Huang  Y, Tsoi  HW, Wong  BH, Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats. Proc Natl Acad Sci U S A. 2005;102:140405. DOIPubMed
  6. Calisher  CH, Childs  JE, Field  HE, Holmes  KV, Schountz  T. Bats: important reservoir hosts of emerging viruses. Clin Microbiol Rev. 2006;19:53145. DOIPubMed
  7. Wibbelt  G, Kurth  A, Yasmum  N, Bannert  M, Nagel  S, Nitsche  A, Discovery of herpesviruses in bats. J Gen Virol. 2007;88:26515. DOIPubMed
  8. Gloza-Rausch  F, Ipsen  A, Seebens  A, Göttsche  M, Panning  M, Drexler  JF, Detection and prevalence patterns of group I coronaviruses in bats, northern Germany. Emerg Infect Dis. 2008;14:62631. DOIPubMed
  9. Linacre  A, Lee  JC. Species determination: the role and use of the cytochrome b gene. Methods Mol Biol. 2005;297:4552.PubMed
  10. Wellehan  JF, Johnson  AJ, Harrach  B, Benko  M, Pessier  AP, Johnson  CM, Detection and analysis of six lizard adenoviruses by consensus primer PCR provides further evidence of a reptilian origin for the atadenoviruses. J Virol. 2004;78:133669. DOIPubMed
  11. van der Hoek  L, Pyrc  K, Jebbink  MF, Vermeulen-Oost  W, Berkhout  RJ, Wolthers  KC, Identification of a new human coronavirus. Nat Med. 2004;10:36873. DOIPubMed
  12. Maeda  K, Hondo  E, Terakawa  J, Kiso  Y, Nakaichi  N, Endoh  D, Isolation of novel adenovirus from fruit bat (Pteropus dasymallus yayeyamae). Emerg Infect Dis. 2008;14:3479. DOIPubMed
  13. Schrenzel  M, Oaks  JL, Rotstein  D, Maalouf  G, Snook  E, Sandfort  C, Characterization of a new species of adenovirus in falcons. J Clin Microbiol. 2005;43:340213. DOIPubMed
  14. Harrach  B. Adenoviruses: general features. In: Mahy BW, van Regenmortel MH, editors. Encyclopedia of virology. Oxford: Elsevier; 2008. p. 1–9.

Main Article

Page created: December 09, 2010
Page updated: December 09, 2010
Page reviewed: December 09, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external