Volume 15, Number 4—April 2009
Dispatch
Concurrent Chikungunya and Dengue Virus Infections during Simultaneous Outbreaks, Gabon, 2007
, Dieudoné Nkoghe, Benjamin Ollomo, Chimène Nze-Nkogue, Pierre Becquart, Gilda Grard, Xavier Pourrut, Rémi N. Charrel, Grégory Moureau, Angélique Ndjoyi-Mbiguino, and Xavier de Lamballerie
Table
Positive test results for CHIKV and DENV-2 among febrile patients, by town, Gabon, 2007*†
| Towns | No. patients tested | No. CHIKV+ | No. DENV-2+ | No. CHIKV+/DENV-2+ |
|---|---|---|---|---|
| Libreville | 686 | 249 | 45 | 6 |
| Ntoum | 3 | 1 | 0 | 0 |
| Kango | 7 | 3 | 0 | 0 |
| Mitzic | 6 | 4 | 0 | 0 |
| Oyem | 45 | 15 | 2 | 1 |
| Minvoul | 7 | 3 | 1 | 1 |
| Cocobeach | 19 | 0 | 6 | 0 |
| Total | 773 | 275 | 54 | 8 |
*CHIKV, chikungunya virus; DENV-2, dengue-2 virus; +, positive.
†RNA was extracted from 50 µL of plasma by using the ABI Prism 6100 Nucleic Acid PrepStation according to the manufacturer’s recommended procedures (Applied Biosystems, Foster City, CA, USA). Fifty-microliter aliquots of extracted RNA were then used in 100-µL High Capacity cDNA synthesis reactions according to the manufacturer’s instructions (Applied Biosystems). Finally, 10 µL of each cDNA reaction was then used as template for 50-µL quantitative PCRs that contained 200 nmol/L of probe and 900 nmol/L of each primer. The quantitative PCRs were then thermo-cycled in a 7500 Real-Time PCR system (Applied Biosystems) according to manufacturer’s recommended procedures. The probe used for the CHIKV, DENV, and DENV-2 assays were FAM-labeled with TAMRA quencher (Applied Biosystems).