Volume 16, Number 2—February 2010
Research
Tropheryma whipplei in Patients with Pneumonia
Table 1
PCR test results of bronchoalveolar lavage specimens that were positive for Tropheryma whipplei DNA, collected from 6 intensive care unit patients in Marseille, France, February 2007–January 2008*
Specimen no. |
16S rDNA
for T. whipplei |
qPCR probe |
Other bacteria identified (16S rDNA primer set) |
||||||
---|---|---|---|---|---|---|---|---|---|
Twhi3 |
Ct |
Twhi2 |
Ct |
Load (copy) |
Bacterium |
Identity, % |
|||
5 | + | + | 20 | + | 21 | 5.105 | – | 99 | |
10 | – | + | 30 | + | 31 | 4,8.102 | Streptococcus pneumoniae | 99 | |
Actinobacillus pleuropneumoniae | 99 | ||||||||
Peptostreptococcus sp. |
99 |
||||||||
82 | – | + | 29 | + | 28 | 5,3.103 | Streptococcus genomosp. C4 | 99 | |
Uncultured | 99 | ||||||||
Streptococcus sp. clone 2.17 | 89 | ||||||||
Streptococcus sanguinis | 99 | ||||||||
Gemella sanguinis | 99 | ||||||||
Leptotrichia sp. | 95 | ||||||||
Haemophilus quentini | 99 | ||||||||
Uncultured Haemophilus sp. | 99 | ||||||||
Peptostreptococcus sp. | 99 | ||||||||
Granulicatella para-adiacens | 95 | ||||||||
Prevotella sp. | 99 | ||||||||
Prevotella melaninogenica | 99 | ||||||||
Cloacibacterium normanense | 93 | ||||||||
Ralstonia solanacearum | 99 | ||||||||
Haemophilus haemolyticus | 99 | ||||||||
Uncultured Arcobacter sp. clone DS126 | 99 | ||||||||
Prevotella salivae | 99 | ||||||||
Streptococcus parasanguis |
99 |
||||||||
86 | – | + | 31 | + | 29 | 7,6.102 | Uncultured Porphyromonas sp. clone 302E06 | 99 | |
Uncultured Capnocytophaga sp. | 95 | ||||||||
Gemella sanguinis | 99 | ||||||||
Streptococcus constellatus | 99 | ||||||||
Prevotella melaninogenica | 91 | ||||||||
Uncultured Porphyromonas sp. | 93 | ||||||||
Haemophilus parainfluenzae | 99 | ||||||||
Uncultured Tannerella sp. | 98 | ||||||||
Prevotella melaninogenica | 99 | ||||||||
Granulicatella para-adiacens |
99 |
||||||||
183 | – | + | 36 | + | 37 | 50 | Staphylococcus epidermidis | 99 | |
Porphyromonas gingivalis | 99 | ||||||||
Pseudomonas sp. | 92 | ||||||||
Eubacterium sp. | 94 | ||||||||
Streptococcus anginosus | 99 | ||||||||
Uncultured Neisseria sp. clone 502G08 | 99 | ||||||||
Treponema sp. | 97 | ||||||||
Clostridium sp. | 97 | ||||||||
Acidovorax sp./Diaphorobacter sp. | 99 | ||||||||
Eubacterium brachy | 99 | ||||||||
Gemella haemolysans | 99 | ||||||||
Mycoplasma orale | 95 | ||||||||
Comamonas denitrificans | 95 | ||||||||
Prevotella tannerae | 98 | ||||||||
Peptostreptococcus micros |
99 |
||||||||
209 | – | + | 35 | + | 35 | 70 | Abiotrophia defectiva | 99 | |
Acidobacteria sp. | 94 | ||||||||
Afipia genosp. 12 | 97 | ||||||||
Sphingomonas sp. | 98 | ||||||||
Pseudomonas stutzeri | 100 |
*qPCR, quantitative PCR; Ct, cycle threshold.