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Volume 17, Number 12—December 2011


Q Fever in Woolsorters, Belgium

Pierre WattiauComments to Author , Eva Boldisova, Rudolf Toman, Marjan Van Esbroeck, Sophie Quoilin, Samia Hammadi, Hervé Tissot-Dupont, Didier Raoult, Jean-Marie Henkinbrant, Mieke Van Hessche, and David Fretin
Author affiliations: Veterinary and Agrochemical Research Centre, Brussels, Belgium (P. Wattiau, M. Van Hessche, D. Fretin); Slovak Academy of Sciences, Bratislava, Slovakia (E. Boldisova, R. Toman); Institute of Tropical Medicine, Antwerp, Belgium (M. Van Esbroeck); Institute of Public Health, Brussels (S. Quoilin, S. Hammadi); Université de la Méditerranée, Marseille, France (H. Tissot-Dupont, D. Raoult); Occupational Health Medicine Group Provikmo, Verviers, Belgium (J.-M. Henkinbrant)

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Table A1

Coxiella burnetii TaqVet quantitative real-time PCR kit results, Belgium, 2007–2009*

Material processed Origin Batch no. Result Ct C. burnetii concentration, GenEq/mg
Goat hair Afghanistan and Russia 07/13528 1.1A + 30.0 103–104
Goat hair Afghanistan and Russia 07/13528 1.1B + 29.0 103104
Goat hair Afghanistan and Russia 07/13528 1.1C + 28.5 103–104
Goat hair Afghanistan 06/11664.5 + 27.0 103–104
Goat hair Asia 08/703.4 + 27.0 103–104
Goat hair Asia 08/703.4 4B + 28.5 103–104
Goat hair Asia 08/703.4 4C + 27.0 103–104
Wool The Netherlands 33945 + 34.0 <102
Wool France and the Netherlands 33961 + 34.0 <102
Wool Italy 33944 + 36.0 <102
Wool Hungary 33954 >40 <10
Wool France and Switzerland 33935 + 36.0 <102

*DNA was extracted by using the QIAamp DNA Stool Mini Kit (QIAGEN, Valencia, CA, USA) from 20-mg of airborne dust samples suspended in 0.5 mL of MiliQ water (Millipore, Billerica, MA, USA). PCR (Laboratoire Service International, Lissieu, France) was conducted according to the manufacturer’s instructions. Ct, cycle threshold; GenEq, genome equivalents; +, positive; –, negative.

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