Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 17, Number 8—August 2011
Dispatch

Fatal Neurologic Disease and Abortion in Mare Infected with Lineage 1 West Nile Virus, South Africa

Marietjie VenterComments to Author , Stacey Human, Stephanie van Niekerk, June Williams, Charmaine van Eeden, and Frank Freeman
Author affiliations: Author affiliations: University of Pretoria, Pretoria, South Africa (M. Venter, S. Human, C. van Eeden, S. van Niekerk, J. Williams); National Institute for Communicable Diseases, Sandringham, Johannesburg, South Africa (M. Venter); Ceres Veterinary Hospital, Western Cape, South Africa (F. Freeman)

Main Article

Figure 2

Maximum-likelihood comparison of a partial section of the E-protein gene (A) and NS5 gene (B) of West Nile virus (WNV) lineage 2 strains isolated in South Africa (SA) in 2010 from a mare with fatal neurologic disease and representative sequences of other WNV lineages from various regions of the world. The lineage 1 strain, SAE75/10, identified in South Africa is indicated by a triangle and arrow. Sequences were aligned with MAFFT version 6 (http://align.bmr.kyushu-u.ac.jp/mafft/software) and edi

Figure 2. Maximum-likelihood comparison of a partial section of the E-protein gene (A) and NS5 gene (B) of West Nile virus (WNV) lineage 2 strains isolated in South Africa (SA) in 2010 from a mare with fatal neurologic disease and representative sequences of other WNV lineages from various regions of the world. The lineage 1 strain, SAE75/10, identified in South Africa is indicated by a triangle and arrow. Sequences were aligned with MAFFT version 6 (http://align.bmr.kyushu-u.ac.jp/mafft/software) and edited with BioEdit version 7.0.9.0 (www.mbio.ncsu.edu/bioedit). Maximum-likelihood midpoint rooted trees were drawn by using PHYML (www.atgc-montpellier.fr/phyml) under 100 bootstrap repetitions and the HKY codon position substitution model and drawn to scale with the bars indicating 0.07 nt substitutions. Only bootstrap values >70 are shown. Reference strains used from GenBank that were most closely related to SA L1: PAH001, Tunisia (AY268133); Ast04-2-824A, Russia (DQ374652); Ast02-2-25, Russia (DQ374653); Ast02-2-692, Russia (DQ411031); Ast01-187, Russia (DQ411035); Ge1b/B, Spain (FJ766331); GE-2o/V, Spain (FJ766332); WNV Italy-1998-equine, Italy (AF404757); WNV15803, Italy (FJ483549); and Ita09, Italy (GU011992).

Main Article

Page created: August 15, 2011
Page updated: August 15, 2011
Page reviewed: August 15, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external