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Volume 17, Number 8—August 2011
Research

Novel Surveillance Network for Norovirus Gastroenteritis Outbreaks, United States1

Everardo Vega, Leslie Barclay, Nicole Gregoricus, Kara Williams, David Lee, and Jan VinjéComments to Author 
Author affiliations: Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (E. Vega, L. Barclay, N. Gregoricus, J. Vinjé); Atlanta Research and Education Foundation, Decatur, Georgia, USA (K. Williams, D. Lee)

Main Article

Table 2

Oligonucleotide primers and probes used for detection and genotype identification of norovirus strains submitted to CaliciNet*

Primer or probe name
 RT-PCR target
 Sequence, 5′ → 3′
 Reference
TVN-L1 ORF2–ORF3 GGG TGT GTT GTG GTG TTG T26VN (29)
L1 ORF2–ORF3 GGG TGT GTT GTG GTG TTG This study
EVP2F P2 (GII.4 specific) GTR CCR CCH ACA GTT GAR TCA This study
EVP2R P2 (GII.4 specific) CCG GGC ATA GTR GAY CTR AAG AA This study
Cap D1 Region D GII TGT CTR STC CCC CAG GAA TG (26)
Cap C Region D GII CCT TYC CAK WTC CCA YGG (26)
Cap D3 Region D GII TGY CTY ITI CCH CAR GAA TGG (26)
Cog 2F ORF1–ORF2 junction (GII) CAR GAR BCN ATG TTY AGR TGG ATG AG (27)
Cog 2R ORF1–ORF2 junction (GII) TCG ACG CCA TCT TCA TTC ACA (27)
Ring 2 ORF1–ORF2 junction (GII) Cy5-TGG GAG GGC GAT CGC AAT CT-BHQ (27)
Ring 1C ORF1–ORF2 junction (GI) FAM-AGA TYG CGI TCI CCT GTC CA-BHQ (28)
Cog 1F ORF1–ORF2 junction (GI) CGY TGG ATG CGI TTY CAT GA (27)
Cog 1R ORF1–ORF2 junction (GI) CTT AGA CGC CAT CAT CAT TYA C (27)

*RT-PCR, reverse transcription PCR; ORF, open reading frame.

Main Article

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Main Article

1Additional members of the CaliciNet participating laboratories who contributed data are listed at the end of this article.

Page created: August 15, 2011
Page updated: August 15, 2011
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The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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