Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 18, Number 2—February 2012
Research

Characterization of Nipah Virus from Outbreaks in Bangladesh, 2008–2010

Michael K. LoComments to Author , Luis Lowe, Kimberly B. Hummel, Hossain M.S. Sazzad, Emily S. Gurley, M. Jahangir Hossain, Stephen P. Luby, David M. Miller, James A. Comer, Pierre E. Rollin, William J. Bellini, and Paul A. Rota
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (M.K. Lo, L. Lowe, K.B. Hummel, D.M. Miller, J.A. Comer, P.E. Rollin, W.J. Bellini, P.A. Rota); International Centre for Diarrheal Disease Research, Bangladesh, Dhaka, Bangladesh (H.M.S. Sazzad, E.S. Gurley, M.J. Hossain, S.P. Luby)

Main Article

Figure 1

Phylogenetic analyses of sequences from the complete Nipah virus N ORF (A) and the 729-nt proposed N ORF genotyping window (B). Tree created with maximum parsimony, close-neighbor-interchange algorithm, 1,000 bootstrap replicates (16). Branch lengths are in units of number of changes over the whole sequence. Available GenBank accession numbers are shown for corresponding sequences. Proposed genotype groupings are indicated by brackets (M, B). ORF, open reading frame; MY, Malaysia; KH, Cambodia;

Figure 1. Phylogenetic analyses of sequences from the complete Nipah virus N ORF (A) and the 729-nt proposed N ORF genotyping window (B). Tree created with maximum parsimony, close-neighbor-interchange algorithm, 1,000 bootstrap replicates (16). Branch lengths are in units of number of changes over the whole sequence. Available GenBank accession numbers are shown for corresponding sequences. Proposed genotype groupings are indicated by brackets (M, B). ORF, open reading frame; MY, Malaysia; KH, Cambodia; BD, Bangladesh; IN, India; HU, human; PI, pig; BA, bat. Scale bars indicate number of sequence changes corresponding to illustrated branch length.

Main Article

References
  1. Chua  KB, Bellini  WJ, Rota  PA, Harcourt  BH, Tamin  A, Lam  SK, Nipah virus: a recently emergent deadly paramyxovirus. Science. 2000;288:14325. DOIPubMedGoogle Scholar
  2. Chua  KB. Nipah virus outbreak in Malaysia. J Clin Virol. 2003;26:26575. DOIPubMedGoogle Scholar
  3. Chadha  MS, Comer  JA, Lowe  L, Rota  PA, Rollin  PE, Bellini  WJ, Nipah virus–associated encephalitis outbreak, Siliguri, India. Emerg Infect Dis. 2006;12:23540.PubMedGoogle Scholar
  4. Arankalle  VA, Bandyopadhyay  BT, Ramdasi  AY, Jadi  R, Patil  DR, Rahman  M, Genomic characterization of Nipah virus, West Bengal, India. Emerg Infect Dis. 2011;17:9079.PubMedGoogle Scholar
  5. Luby  SP, Hossain  MJ, Gurley  ES, Ahmed  B-N, Banu  S, Khan  SU, Recurrent zoonotic transmission of Nipah virus into humans, Bangladesh, 2001–2007. Emerg Infect Dis. 2009;15:122935. DOIPubMedGoogle Scholar
  6. International Centre for Diarrheal Disease Research. Bangladesh. Nipah outbreak in Faridpur District, Bangladesh, 2010. Health and Science Bulletin. 2010;8:611.
  7. Luby  SP, Rahman  M, Hossain  MJ, Blum  LS, Husain  MM, Gurley  E, Foodborne transmission of Nipah virus, Bangladesh. Emerg Infect Dis. 2006;12:188894. DOIPubMedGoogle Scholar
  8. Gurley  ES, Montgomery  JM, Hossain  MJ, Bell  M, Azad  AK, Islam  MR, Person-to-person transmission of Nipah virus in a Bangladeshi community. Emerg Infect Dis. 2007;13:10317.PubMedGoogle Scholar
  9. Luby  SP, Gurley  ES, Hossain  MJ. Transmission of human infections with Nipah virus. Clin Infect Dis. 2009;49:17438. DOIPubMedGoogle Scholar
  10. Lo  MK, Rota  PA. The emergence of Nipah virus, a highly pathogenic paramyxovirus. J Clin Virol. 2008;43:396400. DOIPubMedGoogle Scholar
  11. Harcourt  BH, Lowe  L, Tamin  A, Liu  X, Bankamp  B, Bowden  N, Genetic characterization of Nipah virus, Bangladesh, 2004. Emerg Infect Dis. 2005;11:15947.PubMedGoogle Scholar
  12. Rahman  MA, Hossain  MJ, Sultana  S, Homaira  N, Khan  SU, Rahman  M, Date palm sap linked to Nipah virus outbreak in Bangladesh, 2008. Vector Borne Zoonotic Dis. 2011; [Epub ahead of print]. DOIPubMedGoogle Scholar
  13. Daniels  P, Ksiazek  T, Eaton  BT. Laboratory diagnosis of Nipah and Hendra virus infections. Microbes Infect. 2001;3:28995. DOIPubMedGoogle Scholar
  14. Chomczynski  P, Sacchi  N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987;162:1569. DOIPubMedGoogle Scholar
  15. Halpin  K, Bankamp  B, Harcourt  BH, Bellini  WJ, Rota  PA. Nipah virus conforms to the rule of six in a minigenome replication assay. J Gen Virol. 2004;85:7017. DOIPubMedGoogle Scholar
  16. Tamura  K, Peterson  D, Peterson  N, Stecher  G, Nei  M, Kumar  S. MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;10:27319. DOIPubMedGoogle Scholar
  17. Ong  ST, Yusoff  K, Kho  CL, Abdullah  JO, Tan  WS. Mutagenesis of the nucleocapsid protein of Nipah virus involved in capsid assembly. J Gen Virol. 2009;90:3927. DOIPubMedGoogle Scholar
  18. Chan  YP, Koh  CL, Lam  SK, Wang  LF. Mapping of domains responsible for nucleocapsid protein-phosphoprotein interaction of Henipaviruses. J Gen Virol. 2004;85:167584. DOIPubMedGoogle Scholar
  19. Omi-Furutani  M, Yoneda  M, Fujita  K, Ikeda  F, Kai  C. Novel phosphoprotein-interacting region in Nipah virus nucleocapsid protein and its involvement in viral replication. J Virol. 2010;84:97939. DOIPubMedGoogle Scholar
  20. Rodriguez  JJ, Cruz  CD, Horvath  CM. Identification of the nuclear export signal and STAT-binding domains of the Nipah virus V protein reveals mechanisms underlying interferon evasion. J Virol. 2004;78:535867. DOIPubMedGoogle Scholar
  21. Shaw  ML, Garcia-Sastre  A, Palese  P, Basler  CF. Nipah virus V and W proteins have a common STAT1-binding domain yet inhibit STAT1 activation from the cytoplasmic and nuclear compartments, respectively. J Virol. 2004;78:563341. DOIPubMedGoogle Scholar
  22. Ciancanelli  MJ, Volchkova  VA, Shaw  ML, Volchkov  VE, Basler  CF. Nipah virus sequesters inactive STAT1 in the nucleus via a P gene–encoded mechanism. J Virol. 2009;83:782841. DOIPubMedGoogle Scholar
  23. Rahman  SA, Hassan  SS, Olival  KJ, Mohamed  M, Chang  LY, Hassan  L, Characterization of Nipah virus from naturally infected Pteropus vampyrus bats, Malaysia. Emerg Infect Dis. 2010;16:19903.PubMedGoogle Scholar
  24. Ciancanelli  MJ, Basler  CF. Mutation of YMYL in the Nipah virus matrix protein abrogates budding and alters subcellular localization. J Virol. 2006;80:120708. DOIPubMedGoogle Scholar
  25. Patch  JR, Han  Z, McCarthy  SE, Yan  L, Wang  LF, Harty  RN, The YPLGVG sequence of the Nipah virus matrix protein is required for budding. Virol J. 2008;5:137. DOIPubMedGoogle Scholar
  26. Wang  YE, Park  A, Lake  M, Pentecost  M, Torres  B, Yun  TE, Ubiquitin-regulated nuclear-cytoplasmic trafficking of the Nipah virus matrix protein is important for viral budding. PLoS Pathog. 2010;6:e1001186. DOIPubMedGoogle Scholar
  27. Negrete  OA, Chu  D, Aguilar  HC, Lee  B. Single amino acid changes in the Nipah and Hendra virus attachment glycoproteins distinguish ephrinB2 from ephrinB3 usage. J Virol. 2007;81:1080414. DOIPubMedGoogle Scholar
  28. Bowden  TA, Aricescu  AR, Gilbert  RJ, Grimes  JM, Jones  EY, Stuart  DI. Structural basis of Nipah and Hendra virus attachment to their cell-surface receptor ephrin-B2. Nat Struct Mol Biol. 2008;15:56772. DOIPubMedGoogle Scholar
  29. Poch  O, Blumberg  BM, Bougueleret  L, Tordo  N. Sequence comparison of five polymerases (L proteins) of unsegmented negative-strand RNA viruses: theoretical assignment of functional domains. J Gen Virol. 1990;71:115362. DOIPubMedGoogle Scholar
  30. Harcourt  BH, Tamin  A, Halpin  K, Ksiazek  TG, Rollin  PE, Bellini  WJ, Molecular characterization of the polymerase gene and genomic termini of Nipah virus. Virology. 2001;287:192201. DOIPubMedGoogle Scholar
  31. Rota  PA, Featherstone  DA, Bellini  WJ. Molecular epidemiology of measles virus. Curr Top Microbiol Immunol. 2009;330:12950. DOIPubMedGoogle Scholar
  32. Jin  L, Rima  B, Brown  D, Orvell  C, Tecle  T, Afzal  M, Proposal for genetic characterisation of wild-type mumps strains: preliminary standardisation of the nomenclature. Arch Virol. 2005;150:19039. DOIPubMedGoogle Scholar
  33. Wacharapluesadee  S, Hemachudha  T. Duplex nested RT-PCR for detection of Nipah virus RNA from urine specimens of bats. J Virol Methods. 2007;141:97101. DOIPubMedGoogle Scholar

Main Article

Page created: January 24, 2012
Page updated: January 24, 2012
Page reviewed: January 24, 2012
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external