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Volume 18, Number 5—May 2012
Dispatch

Pigs as Natural Hosts of Dientamoeba fragilis Genotypes Found in Humans

Simone M. CacciòComments to Author , Anna Rosa Sannella, Elisabetta Manuali, Fabio Tosini, Marco Sensi, Daniele Crotti, and Edoardo Pozio
Author affiliations: Istituto Superiore di Sanità, Rome, Italy (S.M. Cacciò, A.R. Sannella, F. Tosini, E. Pozio); Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche, Perugia, Italy (E. Manuali, M. Sensi, D. Crotti)

Main Article

Table 2

Results of the molecular tests for Dientamoeba fragilis applied to pig and human fecal samples, Italy, 2010–2011*

Sample Farm Microscopy 5.8S
18S
ITS1
Real-time PCR Sequence PCR Sequence PCR Sequence
P21 2 + + ND ND ND
P26 2 + + D. fragilis + D. fragilis† + D. fragilis‡
P27 2 + + D. fragilis ND + unclassified
P37 2 + + ND ND ND
P42 3 + + D. fragilis + D. fragilis† + D. fragilis§
P44 3 + + D. fragilis ND + flagellate
P45 3 + + ND ND ND
P50 3 + + ND ND ND
P52 3 + + D. fragilis ND + unclassified
P54 3 + + D. fragilis ND + flagellate
P56 3 + + D. fragilis + D. fragilis† + unclassified
P59 3 + + D. fragilis + D. fragilis† + unclassified
P60 3 + + ND ND ND
P71 4 + + ND ND ND
P75 4 + + ND ND ND
P91 5 + + ND ND ND
P93 5 + + D. fragilis + D. fragilis† + unclassified
P 97 5 + + D. fragilis + D. fragilis† + flagellate
P111 6 + + ND ND ND
P113 6 + + ND ND ND
P116 6 + + ND ND ND
P122 6 + + D. fragilis ND + flagellate
P131 6 + + ND ND ND
P133 7 + + ND ND ND
Pig 1 1 ND + Trichomitus ND
Pig 2 1 ND ND ND
Pig 3 1 ND + Trichomitus ND
Pig 4 1 ND ND ND
Pig 5 1 ND + Trichomitus ND
Pig 6 1 ND ND ND
Pig 7 1 ND ND ND
Pig 8 1 ND ND ND
Pig 9 1 ND ND ND
Pig 10 1 ND ND ND
DF-P1 6 ND ND ND
DF-P2 6 ND ND ND
DF-P3 6 ND ND ND
DF-P4 6 ND ND ND
H1 2 + D. fragilis + D. fragilis† ND
H2 2 ND ND ND
H3 2 + ND ND ND
H4 2 + ND + D. fragilis† ND
H5 5 + ND ND ND
H6 5 + ND + D. fragilis† ND
H7 3 + ND + D. fragilis† ND
H8 3 + ND ND ND
H9 3 ND ND ND
H10 3 + + ND + D. fragilis† ND
H11 3 + + D. fragilis + D. fragilis† ND
H12 3 + ND ND ND
H13 3 + ND ND ND
H14 3 ND ND ND
H15 8 + + D. fragilis + D. fragilis† + D. fragilis#
H16 8 + ND ND ND
H17 9 + D. fragilis + D. fragilis† + D. fragilis#

*ITS, internal transcribed spacer; ND, not done.
†100% identity to AY730405.
‡100% identity to DQ223443, DQ223447, and DQ223455.
§100% identity to DQ223448 and DQ223453.
¶96% identity to AF124610.
#100% identity to DQ223442, DQ223450, DQ223452, DQ223454, DQ223456, and DQ167586.

Main Article

Page created: April 11, 2012
Page updated: April 12, 2012
Page reviewed: April 12, 2012
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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