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Volume 19, Number 1—January 2013
Dispatch

Characterization of Full Genome of Rat Hepatitis E Virus Strain from Vietnam

Tian-Cheng LiComments to Author , Yasushi Ami, Yuriko Suzaki, Shumpei P. Yasuda, Kumiko Yoshimatsu, Jiro Arikawa, Naokazu Takeda, and Wakita Takaji
Author affiliations: Author affiliations: National Institute of Infectious Diseases, Tokyo, Japan (T.-C. Li, Y. Ami, Y. Suzaki, W. Takaji); Graduate School of Medicine, Hokkaido University, Sapporo, Japan (S.P. Yasuda, K. Yoshimatsu, J. Arikawa); Osaka University, Osaka, Japan (N. Takeda)

Main Article

Table 1

Oligonucleotides used in amplifying the complete genome of the rat HEV Vietnam strain, V-105*

Primers Product length, bp
Forward ORF1-F1 (1-21)† 5'-GCAACCCCCGATGGAGACCCA-3'‡
Reverse ORF1-R12 (4149-4171) 5'-GGCGGCCTCGAACTTCTCCTGAA-3'
 §
Forward ORF1-F2 (11-30) 5'-ATGGAGACCCATCAGTATGT-3'†
Reverse ORF1-R1 (431-450) 5'-GTGCAAAAGGAAAGATCAGT-3'
 440
Forward ORF1-F9 (388-408) 5'-AGCTAACAACATCCGCCGTTG-3'
Reverse ORF1-R10 (2197-2217) 5'-TGGGTTCGGTCGAAGGCCTCT-3'†
1,830
Forward ORF1-F16 (2080-2100) 5'-TGCAGCCGTTTATGAGGGAGA-3'
Reverse ORF1-R16 (3055-3075) 5'-CGCCATTCTGTGGGTTCTAGA-3'
996
Forward ORF1-F7 (2990-3009) 5'-GACCCAAGGCAGATCCCTGC-3'†
Reverse ORF1-R12 (4149-4171) 5'-GGCGGCCTCGAACTTCTCCTGAA-3'
1,182
Forward ORF1-F18 (3991-4011) 5'-ATTCACCACAGACGAGCCAGT-3'
Reverse ORF2-R21 (5079-5100) 5'-GGTGATAGCCAATTGGTAAGCT-3'
1,110
Forward F13 (4896-4915) 5'-AATAACACTCTGGGCTGTAG-3'
ReverseTX30SXN 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-3'
 2,092
Forward primer Anchor-1: 5'-CCTCTGAAGGTTCCAGAATCGATAG-3'
Reverse primer ORF1-R14 (276-296) 5'-TAGACCTAGGGTGCGCACCGA-3'
 §
Forward primer Anchor-2: 5'-GAATCGATAGTGAATTCGTG-3'
Reverse primer ORF1-R13 (200-220) 5'-AACACGCTGTACCGGATGCGA-3' 240

*HEV, hepatitis E virus; ORF, open reading frame.
†Numbers in a parentheses show the positions of primers corresponding to the entire genome of rat HEV V-105.
‡Primer designed based on rat HEV (GU345042).
§The PCR product was not detected.

Main Article

Page created: January 04, 2013
Page updated: January 04, 2013
Page reviewed: January 04, 2013
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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