Volume 19, Number 1—January 2013
Dispatch
Characterization of Full Genome of Rat Hepatitis E Virus Strain from Vietnam
Table 1
Oligonucleotides used in amplifying the complete genome of the rat HEV Vietnam strain, V-105*
Primers | Product length, bp |
---|---|
Forward ORF1-F1 (1-21)† 5'-GCAACCCCCGATGGAGACCCA-3'‡ | |
Reverse ORF1-R12 (4149-4171) 5'-GGCGGCCTCGAACTTCTCCTGAA-3' |
§ |
Forward ORF1-F2 (11-30) 5'-ATGGAGACCCATCAGTATGT-3'† | |
Reverse ORF1-R1 (431-450) 5'-GTGCAAAAGGAAAGATCAGT-3' |
440 |
Forward ORF1-F9 (388-408) 5'-AGCTAACAACATCCGCCGTTG-3' | |
Reverse ORF1-R10 (2197-2217) 5'-TGGGTTCGGTCGAAGGCCTCT-3'† |
1,830 |
Forward ORF1-F16 (2080-2100) 5'-TGCAGCCGTTTATGAGGGAGA-3' | |
Reverse ORF1-R16 (3055-3075) 5'-CGCCATTCTGTGGGTTCTAGA-3' |
996 |
Forward ORF1-F7 (2990-3009) 5'-GACCCAAGGCAGATCCCTGC-3'† | |
Reverse ORF1-R12 (4149-4171) 5'-GGCGGCCTCGAACTTCTCCTGAA-3' |
1,182 |
Forward ORF1-F18 (3991-4011) 5'-ATTCACCACAGACGAGCCAGT-3' | |
Reverse ORF2-R21 (5079-5100) 5'-GGTGATAGCCAATTGGTAAGCT-3' |
1,110 |
Forward F13 (4896-4915) 5'-AATAACACTCTGGGCTGTAG-3' | |
ReverseTX30SXN 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-3' |
2,092 |
Forward primer Anchor-1: 5'-CCTCTGAAGGTTCCAGAATCGATAG-3' | |
Reverse primer ORF1-R14 (276-296) 5'-TAGACCTAGGGTGCGCACCGA-3' |
§ |
Forward primer Anchor-2: 5'-GAATCGATAGTGAATTCGTG-3' | |
Reverse primer ORF1-R13 (200-220) 5'-AACACGCTGTACCGGATGCGA-3' | 240 |
*HEV, hepatitis E virus; ORF, open reading frame.
†Numbers in a parentheses show the positions of primers corresponding to the entire genome of rat HEV V-105.
‡Primer designed based on rat HEV (GU345042).
§The PCR product was not detected.