Cell Culture and Electron Microscopy for Identifying Viruses in Diseases of Unknown Cause
Cynthia S. Goldsmith
, Thomas G. Ksiazek, Pierre E. Rollin, James A. Comer, William L. Nicholson, Teresa C.T. Peret, Dean D. Erdman, William J. Bellini, Brian H. Harcourt, Paul A. Rota, Julu Bhatnagar, Michael D. Bowen, Bobbie R. Erickson, Laura K. McMullan, Stuart T. Nichol, Wun-Ju Shieh, Christopher D. Paddock, and Sherif R. Zaki
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (C.S. Goldsmith, P.E. Rollin, J.A. Comer, W.L. Nicholson, T.C.T. Peret, D.D. Erdman, W.J. Bellini, B.H. Harcourt, P.A. Rota, J. Bhatnagar, M.D. Bowen, B.R. Erickson, L.K. McMullan, S.T. Nichol, W.-J. Shieh, C.D. Paddock, S.R. Zaki); University of Texas Medical Branch, Galveston, Texas, USA (T.G. Ksiazek)
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Figure 2
Figure 2. . . A) Extracellular lymphocytic choriomeningitis virus particles (arrow) containing cellular ribosomes. B) West Nile virus particles (arrow) in the lumen of the rough endoplasmic reticulum of an infected cell. Also in the cisternae are smooth membrane vesicles (arrowhead). C) High magnification of Cache Valley virus particles within a Golgi vesicle, showing small surface projections (arrow). D) Extracellular, spherical Homeland virus particles (arrow) with a slightly granular core. Scale bars = 100 nm.
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