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Volume 19, Number 8—August 2013
Research

Comparison of 2 Assays for Diagnosing Rotavirus and Evaluating Vaccine Effectiveness in Children with Gastroenteritis

Jacqueline E. TateComments to Author , Slavica Mijatovic-Rustempasic, Ka Ian Tam, Freda C. Lyde, Daniel C. Payne, Peter Szilagyi, Kathryn Edwards, Mary Allen Staat, Geoffrey A. Weinberg, Caroline B. Hall, James Chappell, Monica McNeal, Jon R. Gentsch, Michael D. Bowen, and Umesh D. Parashar
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (J.E. Tate, S. Mijatovic-Rustempasic, K.I. Tam, F.C. Lyde, D.C. Payne, J.R. Gentsch, M.D. Bowen, U.D. Parashar); University of Rochester School of Medicine and Dentistry, Rochester, New York, USA (P. Szilagyi, G.A. Weinberg, C.B. Hall); Vanderbilt University Medical Center, Nashville, Tennessee, USA (K. Edwards, J. Chappell); Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA (M.A. Staat, M. McNeal)

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Table 2

Comparison of laboratory results in a study of the use of diagnostic assays for rotavirus in children with AGE, 3 New Vaccine Surveillance Network sites (USA), October 2008–October 2009*

Laboratory result Children with AGE
 Healthy controls
Rotavirus EIA+, n = 158 Rotavirus EIA–, n = 490 p value† All, 
n = 500 p value‡ p value§
Virus detected by qRT-PCR
 157 (99)
 65 (13)
 <0.001
24 (5)
 <0.001
 <0.001
Of those with virus detected
Median Ct value (range) 18 (11–40) 36 (23–45) <0.001 32 (21–44) <0.001 0.02
G and P type determined 155 (99) 8 (12) <0.001 12 (50) <0.001 <0.001
Vaccine strain detected 1 (1) 0 0.52 11 (46) <0.001 <0.001

*Values are no. (%) except as indicated. AGE, acute gastroenteritis; EIA, enzyme immunoassay; +, positive; −, negative; qRT-PCR, semiquantitative reverse transcription PCR; Ct, cycle threshold.
†Children with specimens that are EIA+ vs. EIA− for rotavirus.
‡Children with specimens EIA+ for rotavirus vs. healthy children.
§Children with specimens EIA− for rotavirus vs. healthy children.

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Page created: July 02, 2013
Page updated: July 02, 2013
Page reviewed: July 02, 2013
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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